Analysis of the integrant in MyK-103 transgenic mice in which males fail to transmit the integrant.

T. M. Wilkie, R. D. Palmiter

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Males in the transgenic mouse pedigree MyK-103, although fertile, do not transmit the integrant to offspring. The integrant is on chromosome 6 near the T-cell receptor beta-chain locus. It contains four fragments of the plasmid pMK (a metallothionein-thymidine kinase fusion gene) and a 532-base-pair fragment of displaced mouse DNA originating from a previously uncharacterized repetitive DNA family. The integration complex is flanked on either side by a 5-kilobase duplication of mouse DNA normally found in a single copy at this locus. Sequence analysis of the six novel junctions and their donor sequences shows that plasmid-plasmid junctions occurred at patches of limited homology, whereas chromosome-plasmid junctions were nonhomologous.

Original languageEnglish (US)
Pages (from-to)1646-1655
Number of pages10
JournalMolecular and cellular biology
Volume7
Issue number5
DOIs
StatePublished - May 1987

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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