Androgen dependence of specific kallikrein gene family members expressed in rat prostate

J. A. Clements, B. A. Matheson, D. R. Wines, J. M. Brady, R. J. MacDonald, J. W. Funder

Research output: Contribution to journalArticle

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Abstract

We have used oligonucleotide probes specific for members of the rat kallikrein/tonin gene family (PS, S1, S2, S3, K1, and P1) to establish which arginyl esteropeptidase (kallikrein-like) genes are expressed in the prostate. We have also compared the expression and androgen dependence of these genes in prostate, submaxillary gland (SMG) and kidney. Only S3 (tonin-like) and P1 (kallikrein-like) are expressed in the prostate, with S3 very much more abundant. Prostatic S3 mRNA disappears after 8 days castration and is restored to intact levels by dihydrotestosterone (DHT) but not estradiol benzoate (EB) for 8 days. Prostate P1 mRNA levels were similarly but not identically affected. All six genes are expressed in the SMG, with PS (true kallikrein) the most abundant. Levels of PS mRNA in SMG are unaffected by castration, DHT, or EB treatment, although mRNA levels of other kallikrein-like (S1, K1, and P1), tonin (S2), and tonin-like (S3) genes fall 40-60% after castration, and are unaffected or partially restored by DHT and/or EB administration. Only PS and K1 are expressed in the kidney, at much lower levels than in the SMG and unaffected by castration or steroids. These studies thus confirm and extend the concept of tissue specificity of arginyl esteropeptidase gene expression, and further demonstrate that the same gene(s) is differentially regulated by androgens in the rat prostate, SMG, and kidney.

Original languageEnglish (US)
Pages (from-to)16132-16137
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number31
StatePublished - 1988

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Kallikreins
Submandibular Gland
Androgens
Rats
Prostate
Genes
Castration
Messenger RNA
Kidney
Organ Specificity
Oligonucleotide Probes
Dihydrotestosterone
Gene expression
Steroids
Tissue
Gene Expression
tonin
estradiol 3-benzoate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Clements, J. A., Matheson, B. A., Wines, D. R., Brady, J. M., MacDonald, R. J., & Funder, J. W. (1988). Androgen dependence of specific kallikrein gene family members expressed in rat prostate. Journal of Biological Chemistry, 263(31), 16132-16137.

Androgen dependence of specific kallikrein gene family members expressed in rat prostate. / Clements, J. A.; Matheson, B. A.; Wines, D. R.; Brady, J. M.; MacDonald, R. J.; Funder, J. W.

In: Journal of Biological Chemistry, Vol. 263, No. 31, 1988, p. 16132-16137.

Research output: Contribution to journalArticle

Clements, JA, Matheson, BA, Wines, DR, Brady, JM, MacDonald, RJ & Funder, JW 1988, 'Androgen dependence of specific kallikrein gene family members expressed in rat prostate', Journal of Biological Chemistry, vol. 263, no. 31, pp. 16132-16137.
Clements, J. A. ; Matheson, B. A. ; Wines, D. R. ; Brady, J. M. ; MacDonald, R. J. ; Funder, J. W. / Androgen dependence of specific kallikrein gene family members expressed in rat prostate. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 31. pp. 16132-16137.
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