Angiogenesis and morphogenesis of murine fetal distal lung in an allograft model

Margaret A. Schwarz, Fangrong Zhang, John E. Lane, Susan Schachtner, Yangsun Jin, Gail Deutsch, Vaughn Starnes, Bruce R. Pitt

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Neovascularization is crucial to lung morphogenesis; however, factors determining vessel growth and formation are poorly understood. The goal of our study was to develop an allograft model that would include maturation of the distal lung, thereby ultimately allowing us to study alveolar development, including microvascular formation. We transplanted 14-day gestational age embryonic mouse lung primordia subcutaneously into the back of nude mice for 3.5-14 days. Lung morphogenesis and neovascularization were evaluated by light microscopy, in situ hybridization, and immunohistochemical techniques. Embryonic 14-day gestational age control lungs had immature structural features consistent with pseudoglandular stage of lung development. In contrast, 14 days after subcutaneous transplantation of a 14- day gestational age lung, the allograft underwent significant structural morphogenesis and neovascularization. This was demonstrated by continued neovascularization and cellular differentiation, resulting in mature alveoli similar to those noted in the 2-day postnatal neonatal lung. Confirmation of maturation of the allograft was provided by progressive type II epithelial cell differentiation as evidenced by enhanced local expression of mRNA for surfactant protein C and a threefold (P < 0.008) increase in vessel formation as determined by immunocytochemical detection of platelet endothelial cell adhesion molecule-1 expression. Using the tyrosine kinase Flk-1 receptor (flk-1) LacZ transgene embryos, we determined that the neovascularization within the allograft was from the committed embryonic lung endothelium. Therefore, we have developed a defined murine allograft model that can be used to study distal lung development, including neovascularization. The model may be useful when used in conjunction with an altered genetic background (knockout or knock in) of the allograft and has the further decided advantage of bypassing placental barriers for introduction of pharmacological agents or DNA directly into the lung itself.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume278
Issue number5 22-5
StatePublished - May 2000

Fingerprint

Morphogenesis
Allografts
Lung
Gestational Age
CD31 Antigens
Vascular Endothelial Growth Factor Receptor-2
Protein C
Transgenes
Nude Mice
Surface-Active Agents
Endothelium
In Situ Hybridization
Cell Differentiation
Microscopy
Embryonic Structures
Transplantation
Epithelial Cells
Pharmacology
Light
Messenger RNA

Keywords

  • Embryo
  • Lung development
  • Platelet endothelial cell adhesion molecule-1
  • Pulmonary microvasculature
  • Surfactant protein C
  • Vasculogenesis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Schwarz, M. A., Zhang, F., Lane, J. E., Schachtner, S., Jin, Y., Deutsch, G., ... Pitt, B. R. (2000). Angiogenesis and morphogenesis of murine fetal distal lung in an allograft model. American Journal of Physiology - Lung Cellular and Molecular Physiology, 278(5 22-5).

Angiogenesis and morphogenesis of murine fetal distal lung in an allograft model. / Schwarz, Margaret A.; Zhang, Fangrong; Lane, John E.; Schachtner, Susan; Jin, Yangsun; Deutsch, Gail; Starnes, Vaughn; Pitt, Bruce R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 278, No. 5 22-5, 05.2000.

Research output: Contribution to journalArticle

Schwarz, MA, Zhang, F, Lane, JE, Schachtner, S, Jin, Y, Deutsch, G, Starnes, V & Pitt, BR 2000, 'Angiogenesis and morphogenesis of murine fetal distal lung in an allograft model', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 278, no. 5 22-5.
Schwarz, Margaret A. ; Zhang, Fangrong ; Lane, John E. ; Schachtner, Susan ; Jin, Yangsun ; Deutsch, Gail ; Starnes, Vaughn ; Pitt, Bruce R. / Angiogenesis and morphogenesis of murine fetal distal lung in an allograft model. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2000 ; Vol. 278, No. 5 22-5.
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