Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma

C. Romano, D. A. Barrett, Z. Li, A. Pestronk, M. B. Wax

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Purpose. To explore further the potential role autoimmunity may play in the pathogenesis of normal-pressure glaucoma (NPG) in some patients, the authors examined the sera of patients with NPG for the presence of antibodies directed toward retinal antigens. Methods. Using patient sera, immunoblotting was performed on subcellular fractions of retina, purified bovine rhodopsin, and immunoaffinity-purified recombinant human rhodopsin. A chemiluminescence- enzyme-linked immunosorbent assay (ELISA) to detect anti-rhodopsin antibodies was developed and used. Results. A patient with NPG was found to have a high titer of immunoglobulin M-λ antibody against a 40-kd retina-specific glycoprotein antigen subsequently identified as rhodopsin. ELISA analysis conducted on sera from 28 patients with NPG and 26 patients with primary open-angle glaucoma (POAG) revealed highly significant differences in anti- rhodopsin antibody activity between these groups (P < 0.0002, Mann-Whitney test). For example, the majority of patients with NPG (19/28; 68%) had anti- rhodopsin antibody activity higher than the highest value obtained from among 26 age-matched patients with POAG. Conclusions. An elevated anti-rhodopsin antibody count is related to NPG. This may indicate that there is an autoimmune component in the optic neuropathy in these patients. The specific role of these autoantibodies, if any, in the pathogenesis of the disease remains to be determined.

Original languageEnglish (US)
Pages (from-to)1968-1975
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number10
StatePublished - 1995

Fingerprint

Rhodopsin
Glaucoma
Anti-Idiotypic Antibodies
Pressure
Serum
Retina
Enzyme-Linked Immunosorbent Assay
Antigens
Optic Nerve Diseases
Subcellular Fractions
Antibodies
Luminescence
Autoimmunity
Immunoblotting
Autoantibodies
Immunoglobulin M
Glycoproteins

Keywords

  • autoimmune response
  • immunoglobulin
  • immunopathology
  • low-tension glaucoma
  • rhodopsin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Romano, C., Barrett, D. A., Li, Z., Pestronk, A., & Wax, M. B. (1995). Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma. Investigative Ophthalmology and Visual Science, 36(10), 1968-1975.

Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma. / Romano, C.; Barrett, D. A.; Li, Z.; Pestronk, A.; Wax, M. B.

In: Investigative Ophthalmology and Visual Science, Vol. 36, No. 10, 1995, p. 1968-1975.

Research output: Contribution to journalArticle

Romano, C, Barrett, DA, Li, Z, Pestronk, A & Wax, MB 1995, 'Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma', Investigative Ophthalmology and Visual Science, vol. 36, no. 10, pp. 1968-1975.
Romano, C. ; Barrett, D. A. ; Li, Z. ; Pestronk, A. ; Wax, M. B. / Anti-rhodopsin antibodies in sera from patients with normal-pressure glaucoma. In: Investigative Ophthalmology and Visual Science. 1995 ; Vol. 36, No. 10. pp. 1968-1975.
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AB - Purpose. To explore further the potential role autoimmunity may play in the pathogenesis of normal-pressure glaucoma (NPG) in some patients, the authors examined the sera of patients with NPG for the presence of antibodies directed toward retinal antigens. Methods. Using patient sera, immunoblotting was performed on subcellular fractions of retina, purified bovine rhodopsin, and immunoaffinity-purified recombinant human rhodopsin. A chemiluminescence- enzyme-linked immunosorbent assay (ELISA) to detect anti-rhodopsin antibodies was developed and used. Results. A patient with NPG was found to have a high titer of immunoglobulin M-λ antibody against a 40-kd retina-specific glycoprotein antigen subsequently identified as rhodopsin. ELISA analysis conducted on sera from 28 patients with NPG and 26 patients with primary open-angle glaucoma (POAG) revealed highly significant differences in anti- rhodopsin antibody activity between these groups (P < 0.0002, Mann-Whitney test). For example, the majority of patients with NPG (19/28; 68%) had anti- rhodopsin antibody activity higher than the highest value obtained from among 26 age-matched patients with POAG. Conclusions. An elevated anti-rhodopsin antibody count is related to NPG. This may indicate that there is an autoimmune component in the optic neuropathy in these patients. The specific role of these autoantibodies, if any, in the pathogenesis of the disease remains to be determined.

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