Antineuroinflammatory effects of lycopene via activation of adenosine monophosphate-activated protein kinase-α1/heme oxygenase-1 pathways

Hsiao Yun Lin, Bor Ren Huang, Wei Lan Yeh, Chih Hao Lee, Shiang Suo Huang, Chih Ho Lai, Ho Lin, Dah Yuu Lu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Microglia play an important role in the immune defense in the central nervous system. Activation of microglia leads to the production of excessive inflammatory molecules and deleterious consequences, including neuronal death. Lycopene, 1 of the major carotenoids present in tomatoes, has been shown to exert antioxidant properties and to inhibit cancer cell proliferation. However, the effects of lycopene on neuroinflammatory responses in microglia remain unknown. In this study, we investigated the signaling pathways involved in lycopene-inhibited expression of cyclooxygenase (COX)-2 and inflammation mediators in BV-2 microglia, mouse primary cultured microglia, and rat primary cultured microglia. Lycopene inhibited the enhancement of lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-κB) and activator protein 1 (AP-1) DNA binding activity. In the present study, we demonstrated that lycopene inhibits LPS-induced COX-2 expression through heme oxygenase-1 (HO-1) activation. Our results also demonstrate that stimulation with lycopene increases the phosphorylation of liver kinase B1 (LKB1), calmodulin-dependent protein kinase II (CaMKII), and adenosine monophosphate-activated protein kinase (AMPK)-α1. Treatment with AMPK inhibitors effectively antagonized lycopene-stimulated HO-1 expression. Interestingly, we also found that lycopene increased phospho-AMPKα1 accumulation in the nucleus in microglia. Preincubation of cells with HO-1 and AMPK selective pharmacological inhibitors dramatically reversed the inhibitory effect of lycopene on LPS-induced COX-2 and prostaglandin E2 production. Transfection of microglia with HO-1 andAMPKα small interfering RNA (siRNA) also effectively reversed the inhibitory effect of lycopene on LPS-induced COX-2 expression. In a mouse model, lycopene showed significant antineuroinflammatory effects on microglial activation and motor behavior deficits. These findings suggest that lycopene-inhibited LPS-induced COX-2expression is mediated by HO-1 activation through the AMPK pathway. Therefore, lycopene might be useful as a therapeutic agent for the treatment of neuroinflammation-associated disorders.

Original languageEnglish (US)
Pages (from-to)191-202
Number of pages12
JournalNeurobiology of Aging
Volume35
Issue number1
DOIs
StatePublished - Jan 2014

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Heme Oxygenase-1
Adenosine Monophosphate
Protein Kinases
Microglia
Lipopolysaccharides
Cyclooxygenase 2
lycopene
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Inflammation Mediators
Transcription Factor AP-1
Carotenoids
Lycopersicon esculentum
Protein Kinase Inhibitors
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Small Interfering RNA
Transfection
Phosphotransferases

Keywords

  • AMPKα1
  • COX-2
  • Heme oxygenase-1
  • Lycopene
  • Microglia

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Aging
  • Developmental Biology
  • Geriatrics and Gerontology

Cite this

Antineuroinflammatory effects of lycopene via activation of adenosine monophosphate-activated protein kinase-α1/heme oxygenase-1 pathways. / Lin, Hsiao Yun; Huang, Bor Ren; Yeh, Wei Lan; Lee, Chih Hao; Huang, Shiang Suo; Lai, Chih Ho; Lin, Ho; Lu, Dah Yuu.

In: Neurobiology of Aging, Vol. 35, No. 1, 01.2014, p. 191-202.

Research output: Contribution to journalArticle

Lin, Hsiao Yun ; Huang, Bor Ren ; Yeh, Wei Lan ; Lee, Chih Hao ; Huang, Shiang Suo ; Lai, Chih Ho ; Lin, Ho ; Lu, Dah Yuu. / Antineuroinflammatory effects of lycopene via activation of adenosine monophosphate-activated protein kinase-α1/heme oxygenase-1 pathways. In: Neurobiology of Aging. 2014 ; Vol. 35, No. 1. pp. 191-202.
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