Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides

Mark Schaefer, Marina R. Picciotto, Thane Kreiner, Rashad Rudolf Kaldany, Ronald Taussig, Richard H. Scheller

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

The neuroactive peptide Phe-Met-Arg-Phe-NH2 (FMRFamide) has a variety of effects on both mammalian and invertebrate tissues; moreover, FMRFamide-like immunoreactivity is found throughout the animal kingdom. Here we describe the isolation and characterization of a cDNA clone from an Aplysia abdominal ganglion cDNA library that encodes a precursor protein that may give rise to as many as 19 individual FMRFamide peptides. Nearly all of the FMRF sequences are flanked on the amino terminus by Lys-Arg residues and on the carboxy terminus by Gly-Lys residues,suggesting that the single lysine residues function to signal cleavage by processing enzymes. The gene is present in a single copy per haploid genome and gives rise to multiple transcripts, at least some of which appear to arise through alternate RNA splicing. Immunohistochemical analysis suggests that the peptide is present in many neurons throughout the Aplysia nervous system and that these neurons send processes to a variety of different tissues.

Original languageEnglish (US)
Pages (from-to)457-467
Number of pages11
JournalCell
Volume41
Issue number2
DOIs
StatePublished - 1985

Fingerprint

FMRFamide
Aplysia
Gene encoding
Neuropeptides
Neurons
Genes
Peptides
glycyllysine
Tissue
RNA Splicing
Protein Precursors
Haploidy
Alternative Splicing
Neurology
Invertebrates
Gene Library
Ganglia
Nervous System
Lysine
Animals

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Schaefer, M., Picciotto, M. R., Kreiner, T., Kaldany, R. R., Taussig, R., & Scheller, R. H. (1985). Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides. Cell, 41(2), 457-467. https://doi.org/10.1016/S0092-8674(85)80019-2

Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides. / Schaefer, Mark; Picciotto, Marina R.; Kreiner, Thane; Kaldany, Rashad Rudolf; Taussig, Ronald; Scheller, Richard H.

In: Cell, Vol. 41, No. 2, 1985, p. 457-467.

Research output: Contribution to journalArticle

Schaefer, M, Picciotto, MR, Kreiner, T, Kaldany, RR, Taussig, R & Scheller, RH 1985, 'Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides', Cell, vol. 41, no. 2, pp. 457-467. https://doi.org/10.1016/S0092-8674(85)80019-2
Schaefer, Mark ; Picciotto, Marina R. ; Kreiner, Thane ; Kaldany, Rashad Rudolf ; Taussig, Ronald ; Scheller, Richard H. / Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides. In: Cell. 1985 ; Vol. 41, No. 2. pp. 457-467.
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