TY - JOUR
T1 - Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides
AU - Schaefer, Mark
AU - Picciotto, Marina R.
AU - Kreiner, Thane
AU - Kaldany, Rashad Rudolf
AU - Taussig, Ronald
AU - Scheller, Richard H.
N1 - Funding Information:
We are grateful to A. C. Mahon and J. R. Nambu for providing the ring and genomic libraries. The FMRFamide antibody was generously supplied by Drs. Eckard Weber and Jack D. Barchas. We also thank Corey Goodman for his critical reading of the manuscript. R. R. K. is a National Institutes of Health postdoctoral fellow and T. K. is a National Science Foundation predoctoral student. This work is supported by grants to R. H. S. from the National Institutes of Health and National Science Foundation. R. H. S. is an Alfred R Sloan Fellow and a McKnight Foundation Scholar, and is the recipient of the March of Dimes Basil O'Connor award.
PY - 1985/6
Y1 - 1985/6
N2 - The neuroactive peptide Phe-Met-Arg-Phe-NH2 (FMRFamide) has a variety of effects on both mammalian and invertebrate tissues; moreover, FMRFamide-like immunoreactivity is found throughout the animal kingdom. Here we describe the isolation and characterization of a cDNA clone from an Aplysia abdominal ganglion cDNA library that encodes a precursor protein that may give rise to as many as 19 individual FMRFamide peptides. Nearly all of the FMRF sequences are flanked on the amino terminus by Lys-Arg residues and on the carboxy terminus by Gly-Lys residues,suggesting that the single lysine residues function to signal cleavage by processing enzymes. The gene is present in a single copy per haploid genome and gives rise to multiple transcripts, at least some of which appear to arise through alternate RNA splicing. Immunohistochemical analysis suggests that the peptide is present in many neurons throughout the Aplysia nervous system and that these neurons send processes to a variety of different tissues.
AB - The neuroactive peptide Phe-Met-Arg-Phe-NH2 (FMRFamide) has a variety of effects on both mammalian and invertebrate tissues; moreover, FMRFamide-like immunoreactivity is found throughout the animal kingdom. Here we describe the isolation and characterization of a cDNA clone from an Aplysia abdominal ganglion cDNA library that encodes a precursor protein that may give rise to as many as 19 individual FMRFamide peptides. Nearly all of the FMRF sequences are flanked on the amino terminus by Lys-Arg residues and on the carboxy terminus by Gly-Lys residues,suggesting that the single lysine residues function to signal cleavage by processing enzymes. The gene is present in a single copy per haploid genome and gives rise to multiple transcripts, at least some of which appear to arise through alternate RNA splicing. Immunohistochemical analysis suggests that the peptide is present in many neurons throughout the Aplysia nervous system and that these neurons send processes to a variety of different tissues.
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U2 - 10.1016/S0092-8674(85)80019-2
DO - 10.1016/S0092-8674(85)80019-2
M3 - Article
C2 - 3838698
AN - SCOPUS:0022080810
SN - 0092-8674
VL - 41
SP - 457
EP - 467
JO - Cell
JF - Cell
IS - 2
ER -