Aquaporins in rat pancreatic interlobular ducts

Shigeru B H Ko, Satoru Naruse, Motoji Kitagawa, Hiroshi Ishiguro, Sonoko Furuya, Nobumasa Mizuno, Youxue Wang, Toshiyuki Yoshikawa, Atsushi Suzuki, Shoko Shimano, Tetsuo Hayakawa

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The aquaporin (AQP) family of water channels is distributed ubiquitously in many epithelia and plays a fundamental role in transmembrane water transport. The aim of this study is to identify the water transport pathway in pancreatic duct cells where most of the HCO3 --rich fluid originates. Using digital videomicroscopy, we measured the osmotic water permeability (Pf) of pancreatic duct epithelium by exposing isolated rat interlobular ducts to the hypotonic solution (145 mosM). To identify mRNA and protein of AQPs expressed in duct cells, we conducted RT-PCR analysis and immunohistochemistry of the isolated duct and pancreas. The calculated Pf (160-230 μm/s) of the isolated ducts was significantly reduced to 16-35 μm/s by 80-90% with either basolateral or luminal applications of HgCl2. Fluid secretion evoked by secretin was almost completely abolished by a basolateral or luminal application of HgCl2. A large amount of AQP1 and small amounts of AQP5 transcripts were detected in the isolated duct cells by RT-PCR. AQP1, but not AQP5, immunoreactivity was present in both luminal and basolateral membranes of the interlobular duct cells. Mercury-sensitive water channels are present in both luminal and basolateral membranes of rat pancreatic ducts. AQP1 of the known AQPs appears to be the main water pathway in interlobular ducts.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume282
Issue number2 45-2
StatePublished - 2002

Fingerprint

Aquaporins
Pancreatic Ducts
Mercuric Chloride
Water
Epithelium
Hypotonic Solutions
Fluids and Secretions
Video Microscopy
Polymerase Chain Reaction
Secretin
Membranes
Mercury
Pancreas
Permeability
Immunohistochemistry
Messenger RNA
Proteins

Keywords

  • Fluid secretion
  • Isolated pancreatic duct
  • Mercury-sensitive water channel
  • Water permeability

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Ko, S. B. H., Naruse, S., Kitagawa, M., Ishiguro, H., Furuya, S., Mizuno, N., ... Hayakawa, T. (2002). Aquaporins in rat pancreatic interlobular ducts. American Journal of Physiology - Gastrointestinal and Liver Physiology, 282(2 45-2).

Aquaporins in rat pancreatic interlobular ducts. / Ko, Shigeru B H; Naruse, Satoru; Kitagawa, Motoji; Ishiguro, Hiroshi; Furuya, Sonoko; Mizuno, Nobumasa; Wang, Youxue; Yoshikawa, Toshiyuki; Suzuki, Atsushi; Shimano, Shoko; Hayakawa, Tetsuo.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 282, No. 2 45-2, 2002.

Research output: Contribution to journalArticle

Ko, SBH, Naruse, S, Kitagawa, M, Ishiguro, H, Furuya, S, Mizuno, N, Wang, Y, Yoshikawa, T, Suzuki, A, Shimano, S & Hayakawa, T 2002, 'Aquaporins in rat pancreatic interlobular ducts', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 282, no. 2 45-2.
Ko SBH, Naruse S, Kitagawa M, Ishiguro H, Furuya S, Mizuno N et al. Aquaporins in rat pancreatic interlobular ducts. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002;282(2 45-2).
Ko, Shigeru B H ; Naruse, Satoru ; Kitagawa, Motoji ; Ishiguro, Hiroshi ; Furuya, Sonoko ; Mizuno, Nobumasa ; Wang, Youxue ; Yoshikawa, Toshiyuki ; Suzuki, Atsushi ; Shimano, Shoko ; Hayakawa, Tetsuo. / Aquaporins in rat pancreatic interlobular ducts. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 282, No. 2 45-2.
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