Arachidonic acid epoxygenase: Structural characterization and quantification of epoxyeicosatrienoates in plasma

Armando Karara, Shouzou Wei, David Spady, Larry Swift, Jorge H. Capdevila, J R Falck

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 ± 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 ± 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 ± 0.1 and 1.9 ± 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.

Original languageEnglish (US)
Pages (from-to)1320-1325
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
StatePublished - Feb 14 1992

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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