TY - JOUR
T1 - Arachidonic acid epoxygenase
T2 - Structural characterization and quantification of epoxyeicosatrienoates in plasma
AU - Karara, Armando
AU - Wei, Shouzou
AU - Spady, David
AU - Swift, Larry
AU - Capdevila, Jorge H.
AU - Falck, J R
PY - 1992/2/14
Y1 - 1992/2/14
N2 - Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 ± 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 ± 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 ± 0.1 and 1.9 ± 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.
AB - Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 ± 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 ± 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 ± 0.1 and 1.9 ± 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.
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U2 - 10.1016/0006-291X(92)91877-S
DO - 10.1016/0006-291X(92)91877-S
M3 - Article
C2 - 1540175
AN - SCOPUS:0026523719
SN - 0006-291X
VL - 182
SP - 1320
EP - 1325
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -