Architecture of the large subunit of the mammalian mitochondrial ribosome

Basil J. Greber, Daniel Boehringer, Alexander Leitner, Philipp Bieri, Felix Voigts-Hoffmann, Jan P. Erzberger, Marc Leibundgut, Ruedi Aebersold, Nenad Ban

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins. Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4.9 Å resolution. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site. This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain.

Original languageEnglish (US)
Pages (from-to)515-519
Number of pages5
JournalNature
Volume505
Issue number7484
DOIs
StatePublished - Jan 10 2014

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Mitochondria
Cryoelectron Microscopy
Ribosomal RNA
Ribosomal Proteins
Oxidative Phosphorylation
Eukaryotic Cells
Electron Transport
Ribosomes
Organelles
Mass Spectrometry
Proteins
Swine
Genome
Peptides
Membranes
Mitochondrial Ribosomes

ASJC Scopus subject areas

  • General

Cite this

Greber, B. J., Boehringer, D., Leitner, A., Bieri, P., Voigts-Hoffmann, F., Erzberger, J. P., ... Ban, N. (2014). Architecture of the large subunit of the mammalian mitochondrial ribosome. Nature, 505(7484), 515-519. https://doi.org/10.1038/nature12890

Architecture of the large subunit of the mammalian mitochondrial ribosome. / Greber, Basil J.; Boehringer, Daniel; Leitner, Alexander; Bieri, Philipp; Voigts-Hoffmann, Felix; Erzberger, Jan P.; Leibundgut, Marc; Aebersold, Ruedi; Ban, Nenad.

In: Nature, Vol. 505, No. 7484, 10.01.2014, p. 515-519.

Research output: Contribution to journalArticle

Greber, BJ, Boehringer, D, Leitner, A, Bieri, P, Voigts-Hoffmann, F, Erzberger, JP, Leibundgut, M, Aebersold, R & Ban, N 2014, 'Architecture of the large subunit of the mammalian mitochondrial ribosome', Nature, vol. 505, no. 7484, pp. 515-519. https://doi.org/10.1038/nature12890
Greber BJ, Boehringer D, Leitner A, Bieri P, Voigts-Hoffmann F, Erzberger JP et al. Architecture of the large subunit of the mammalian mitochondrial ribosome. Nature. 2014 Jan 10;505(7484):515-519. https://doi.org/10.1038/nature12890
Greber, Basil J. ; Boehringer, Daniel ; Leitner, Alexander ; Bieri, Philipp ; Voigts-Hoffmann, Felix ; Erzberger, Jan P. ; Leibundgut, Marc ; Aebersold, Ruedi ; Ban, Nenad. / Architecture of the large subunit of the mammalian mitochondrial ribosome. In: Nature. 2014 ; Vol. 505, No. 7484. pp. 515-519.
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