Aromatization of androstenedione by human adipose tissue stromal cells in monolayer culture

G. E. Ackerman, M. E. Smith, C. R. Mendelson, P. C. MacDonald, E. R. Simpson

Research output: Contribution to journalArticle

345 Citations (Scopus)

Abstract

Stromal cells and adipocytes were separated after collagenase treatment of adipose tissue obtained from women undergoing elective surgery, and these cells were used to study aromatization of [ 3H]androstenedione in vitro. Aromatization activity was estimated either 1) by determining the incorporation of tritium from [1- 3H]androstenedione into [ 3H]water or else 2) by determining the formation of [ 3H]estrone (E 1) and [ 3H]estradiol (E 2) from [1,2,6,7- 3H]androstenedione. It was established that only 13% of the aromatase activity of adipose tissue resided in the adipocyte fraction, whereas 87% of the aromatase activity was in the stromal/vascular fraction. Subsequent studies of aromatization were conducted utilizing stromal/vascular cells grown to confluence in monolayer culture. In such cells, the formation of [ 3H]E 2 was slower initially but increased with time, and after 48 h of incubation, the amount of [ 3H]E 2 produced exceeded that of [ 3H]E 1. The rate of [ 3H]E 1 formation, as a function of [ 3H]androstenedione concentration, followed Michaeilis-Menten kinetics. The V(max) ranged from 0.8-3.0 pmol mg -1 cell protein 6 h -1 in stromal cells from sc adipose tissue and ranged from 0.16-0.67 pmol mg -1 cell protein 6 h -1 in cells from omental adipose tissue. The apparent K(m) for [ 3H]androstenedione in stromal cells derived from both omental and sc tissue was the same, i.e. about 25 nM. We conclude that the ability of human adipose tissue to form estrogen is not a function primarily of the adipocyte but rather resides principally in the cells of the stroma.

Original languageEnglish (US)
Pages (from-to)412-417
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume53
Issue number2
StatePublished - 1981

Fingerprint

Aromatization
Androstenedione
Stromal Cells
Adipose Tissue
Monolayers
Tissue
Adipocytes
Aromatase
Blood Vessels
Tritium
Estrone
Collagenases
Cell culture
Surgery
Estradiol
Estrogens
Proteins
Cells
Kinetics
Water

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Aromatization of androstenedione by human adipose tissue stromal cells in monolayer culture. / Ackerman, G. E.; Smith, M. E.; Mendelson, C. R.; MacDonald, P. C.; Simpson, E. R.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 53, No. 2, 1981, p. 412-417.

Research output: Contribution to journalArticle

Ackerman, G. E. ; Smith, M. E. ; Mendelson, C. R. ; MacDonald, P. C. ; Simpson, E. R. / Aromatization of androstenedione by human adipose tissue stromal cells in monolayer culture. In: Journal of Clinical Endocrinology and Metabolism. 1981 ; Vol. 53, No. 2. pp. 412-417.
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