Assessment of HER-2/neun status in breast cancer: Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard

Sijian Wang, M. Hossein Saboorian, Eugene P. Frenkel, Barbara B. Haley, Momin T. Siddiqui, Sefik Gokaslan, Frank H. Wians, Linda Hynan, Raheela Ashfaq

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0% and 88% vs 85.7% and 71%, respectively), with only a slight decrease in specificity (93% vs 96%, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status.

Original languageEnglish (US)
Pages (from-to)495-503
Number of pages9
JournalAmerican Journal of Clinical Pathology
Volume116
Issue number4
DOIs
StatePublished - 2001

Fingerprint

Fluorescence In Situ Hybridization
Breast Neoplasms
erbB-2 Genes
Gene Amplification
ROC Curve
Formaldehyde
Retrospective Studies
Staining and Labeling

Keywords

  • ACIS
  • Breast cancer
  • ErbB-2
  • Fluorescence in situ hybridization
  • HER-2/neu
  • Image analysis
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Assessment of HER-2/neun status in breast cancer : Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard. / Wang, Sijian; Saboorian, M. Hossein; Frenkel, Eugene P.; Haley, Barbara B.; Siddiqui, Momin T.; Gokaslan, Sefik; Wians, Frank H.; Hynan, Linda; Ashfaq, Raheela.

In: American Journal of Clinical Pathology, Vol. 116, No. 4, 2001, p. 495-503.

Research output: Contribution to journalArticle

@article{97c29469101c40f48cc64e03664b9f02,
title = "Assessment of HER-2/neun status in breast cancer: Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard",
abstract = "This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0{\%} and 88{\%} vs 85.7{\%} and 71{\%}, respectively), with only a slight decrease in specificity (93{\%} vs 96{\%}, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status.",
keywords = "ACIS, Breast cancer, ErbB-2, Fluorescence in situ hybridization, HER-2/neu, Image analysis, Immunohistochemistry",
author = "Sijian Wang and Saboorian, {M. Hossein} and Frenkel, {Eugene P.} and Haley, {Barbara B.} and Siddiqui, {Momin T.} and Sefik Gokaslan and Wians, {Frank H.} and Linda Hynan and Raheela Ashfaq",
year = "2001",
doi = "10.1309/TMUW-G4WB-LXJ2-FUDN",
language = "English (US)",
volume = "116",
pages = "495--503",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "4",

}

TY - JOUR

T1 - Assessment of HER-2/neun status in breast cancer

T2 - Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard

AU - Wang, Sijian

AU - Saboorian, M. Hossein

AU - Frenkel, Eugene P.

AU - Haley, Barbara B.

AU - Siddiqui, Momin T.

AU - Gokaslan, Sefik

AU - Wians, Frank H.

AU - Hynan, Linda

AU - Ashfaq, Raheela

PY - 2001

Y1 - 2001

N2 - This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0% and 88% vs 85.7% and 71%, respectively), with only a slight decrease in specificity (93% vs 96%, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status.

AB - This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0% and 88% vs 85.7% and 71%, respectively), with only a slight decrease in specificity (93% vs 96%, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status.

KW - ACIS

KW - Breast cancer

KW - ErbB-2

KW - Fluorescence in situ hybridization

KW - HER-2/neu

KW - Image analysis

KW - Immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0035542711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035542711&partnerID=8YFLogxK

U2 - 10.1309/TMUW-G4WB-LXJ2-FUDN

DO - 10.1309/TMUW-G4WB-LXJ2-FUDN

M3 - Article

C2 - 11601134

AN - SCOPUS:0035542711

VL - 116

SP - 495

EP - 503

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 4

ER -