Assessment of HER-2/neun status in breast cancer: Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard

Sijian Wang, M. Hossein Saboorian, Eugene P. Frenkel, Barbara B. Haley, Momin T. Siddiqui, Sefik Gokaslan, Frank H. Wians, Linda Hynan, Raheela Ashfaq

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0% and 88% vs 85.7% and 71%, respectively), with only a slight decrease in specificity (93% vs 96%, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status.

Original languageEnglish (US)
Pages (from-to)495-503
Number of pages9
JournalAmerican journal of clinical pathology
Volume116
Issue number4
DOIs
StatePublished - Dec 1 2001

Keywords

  • ACIS
  • Breast cancer
  • ErbB-2
  • Fluorescence in situ hybridization
  • HER-2/neu
  • Image analysis
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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