Abstract
Purpose. To evaluate the role of nm23 gene expression in the development of metastases of human uveal melanomas in an animal model. Methods. Seven human uveal melanoma cell lines and two murine skill melanoma cell lines were subjected to Northern blot analysis for the detection of nm23-H1 mRNA and to immunohistochemistry to detect nm23 antigen. Each tumor cell line was transplanted intracamerally into nude mice, and the metastatic behavior was evaluated by histopathologic analysis of the livers and by determining host survival times. Results. There was a strong inverse correlation between the levels of nm23 mRNA expression and nm23 antigen expression and the development of metastases of all seven human uveal melanomas and both murine skin melanomas transplanted intracamerally. Host survival time also was correlated with the degree of nm23 gene expression. Conclusions. The expression of nm23 mRNA and nm23 antigen in human uveal melanomas is correlated closely with reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator of malignancy and survival in patients with uveal melanomas.
Original language | English (US) |
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Pages (from-to) | 2293-2301 |
Number of pages | 9 |
Journal | Investigative Ophthalmology and Visual Science |
Volume | 37 |
Issue number | 11 |
State | Published - Oct 1996 |
Keywords
- NM23
- melanoma
- metastasis
- tumor suppressor gene
- uvea
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience