Association between NM23-H1 gene expression and metastasis of human uveal melanoma in an animal model

Ding Ma, Gregorius P. Luyten, Theo M. Luider, Martine J. Jager, Jerry Y. Niederkorn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose. To evaluate the role of nm23 gene expression in the development of metastases of human uveal melanomas in an animal model. Methods. Seven human uveal melanoma cell lines and two murine skill melanoma cell lines were subjected to Northern blot analysis for the detection of nm23-H1 mRNA and to immunohistochemistry to detect nm23 antigen. Each tumor cell line was transplanted intracamerally into nude mice, and the metastatic behavior was evaluated by histopathologic analysis of the livers and by determining host survival times. Results. There was a strong inverse correlation between the levels of nm23 mRNA expression and nm23 antigen expression and the development of metastases of all seven human uveal melanomas and both murine skin melanomas transplanted intracamerally. Host survival time also was correlated with the degree of nm23 gene expression. Conclusions. The expression of nm23 mRNA and nm23 antigen in human uveal melanomas is correlated closely with reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator of malignancy and survival in patients with uveal melanomas.

Original languageEnglish (US)
Pages (from-to)2293-2301
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number11
StatePublished - Oct 1996

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Animal Models
Neoplasm Metastasis
Gene Expression
Antigens
Messenger RNA
Survival
Melanoma
Cell Line
Human Development
Tumor Cell Line
Nude Mice
Northern Blotting
Immunohistochemistry
Uveal melanoma
Skin
Liver
Neoplasms

Keywords

  • melanoma
  • metastasis
  • NM23
  • tumor suppressor gene
  • uvea

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Association between NM23-H1 gene expression and metastasis of human uveal melanoma in an animal model. / Ma, Ding; Luyten, Gregorius P.; Luider, Theo M.; Jager, Martine J.; Niederkorn, Jerry Y.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 11, 10.1996, p. 2293-2301.

Research output: Contribution to journalArticle

Ma, Ding ; Luyten, Gregorius P. ; Luider, Theo M. ; Jager, Martine J. ; Niederkorn, Jerry Y. / Association between NM23-H1 gene expression and metastasis of human uveal melanoma in an animal model. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 11. pp. 2293-2301.
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abstract = "Purpose. To evaluate the role of nm23 gene expression in the development of metastases of human uveal melanomas in an animal model. Methods. Seven human uveal melanoma cell lines and two murine skill melanoma cell lines were subjected to Northern blot analysis for the detection of nm23-H1 mRNA and to immunohistochemistry to detect nm23 antigen. Each tumor cell line was transplanted intracamerally into nude mice, and the metastatic behavior was evaluated by histopathologic analysis of the livers and by determining host survival times. Results. There was a strong inverse correlation between the levels of nm23 mRNA expression and nm23 antigen expression and the development of metastases of all seven human uveal melanomas and both murine skin melanomas transplanted intracamerally. Host survival time also was correlated with the degree of nm23 gene expression. Conclusions. The expression of nm23 mRNA and nm23 antigen in human uveal melanomas is correlated closely with reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator of malignancy and survival in patients with uveal melanomas.",
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T1 - Association between NM23-H1 gene expression and metastasis of human uveal melanoma in an animal model

AU - Ma, Ding

AU - Luyten, Gregorius P.

AU - Luider, Theo M.

AU - Jager, Martine J.

AU - Niederkorn, Jerry Y.

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N2 - Purpose. To evaluate the role of nm23 gene expression in the development of metastases of human uveal melanomas in an animal model. Methods. Seven human uveal melanoma cell lines and two murine skill melanoma cell lines were subjected to Northern blot analysis for the detection of nm23-H1 mRNA and to immunohistochemistry to detect nm23 antigen. Each tumor cell line was transplanted intracamerally into nude mice, and the metastatic behavior was evaluated by histopathologic analysis of the livers and by determining host survival times. Results. There was a strong inverse correlation between the levels of nm23 mRNA expression and nm23 antigen expression and the development of metastases of all seven human uveal melanomas and both murine skin melanomas transplanted intracamerally. Host survival time also was correlated with the degree of nm23 gene expression. Conclusions. The expression of nm23 mRNA and nm23 antigen in human uveal melanomas is correlated closely with reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator of malignancy and survival in patients with uveal melanomas.

AB - Purpose. To evaluate the role of nm23 gene expression in the development of metastases of human uveal melanomas in an animal model. Methods. Seven human uveal melanoma cell lines and two murine skill melanoma cell lines were subjected to Northern blot analysis for the detection of nm23-H1 mRNA and to immunohistochemistry to detect nm23 antigen. Each tumor cell line was transplanted intracamerally into nude mice, and the metastatic behavior was evaluated by histopathologic analysis of the livers and by determining host survival times. Results. There was a strong inverse correlation between the levels of nm23 mRNA expression and nm23 antigen expression and the development of metastases of all seven human uveal melanomas and both murine skin melanomas transplanted intracamerally. Host survival time also was correlated with the degree of nm23 gene expression. Conclusions. The expression of nm23 mRNA and nm23 antigen in human uveal melanomas is correlated closely with reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator of malignancy and survival in patients with uveal melanomas.

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