Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system

Zhiyuan Shen, Paige E. Pardington-Purtymun, Jarmon C. Comeaux, Robert K. Moyzis, David J. Chen

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Abstract

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the 'bait' in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53- mediated pathway(s), and ubiquitination interact through human UBE2I.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalGenomics
Volume37
Issue number2
DOIs
StatePublished - Oct 15 1996

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SUMO-1 Protein
Rad51 Recombinase
Two-Hybrid System Techniques
Yeasts
Ubiquitins
Ubiquitin-Conjugating Enzymes
Cyclins
Double-Stranded DNA Breaks
Ubiquitination
Cell Cycle Checkpoints
S Phase
DNA Repair
Cell Division
Genetic Recombination
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Shen, Z., Pardington-Purtymun, P. E., Comeaux, J. C., Moyzis, R. K., & Chen, D. J. (1996). Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system. Genomics, 37(2), 183-186. https://doi.org/10.1006/geno.1996.0540

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system. / Shen, Zhiyuan; Pardington-Purtymun, Paige E.; Comeaux, Jarmon C.; Moyzis, Robert K.; Chen, David J.

In: Genomics, Vol. 37, No. 2, 15.10.1996, p. 183-186.

Research output: Contribution to journalArticle

Shen, Z, Pardington-Purtymun, PE, Comeaux, JC, Moyzis, RK & Chen, DJ 1996, 'Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system', Genomics, vol. 37, no. 2, pp. 183-186. https://doi.org/10.1006/geno.1996.0540
Shen Z, Pardington-Purtymun PE, Comeaux JC, Moyzis RK, Chen DJ. Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system. Genomics. 1996 Oct 15;37(2):183-186. https://doi.org/10.1006/geno.1996.0540
Shen, Zhiyuan ; Pardington-Purtymun, Paige E. ; Comeaux, Jarmon C. ; Moyzis, Robert K. ; Chen, David J. / Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system. In: Genomics. 1996 ; Vol. 37, No. 2. pp. 183-186.
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