Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice

Veronica S. Valentinuzzi, Daniel E. Kolker, Martha Hotz Vitaterna, Kazuhiro Shimomura, Andrew Whiteley, Sharon Low-Zeddies, Fred W. Turek, Elenice A M Ferrari, Richard Paylor, Joseph S. Takahashi

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.

Original languageEnglish (US)
Pages (from-to)391-403
Number of pages13
JournalLearning and Memory
Volume5
Issue number4-5
StatePublished - 1998

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Quantitative Trait Loci
Freezing
Fear
Chromosomes, Human, Pair 8
Automation
Computer Systems
Hand
Observation
Learning
Efficiency
Conditioning (Psychology)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice. / Valentinuzzi, Veronica S.; Kolker, Daniel E.; Vitaterna, Martha Hotz; Shimomura, Kazuhiro; Whiteley, Andrew; Low-Zeddies, Sharon; Turek, Fred W.; Ferrari, Elenice A M; Paylor, Richard; Takahashi, Joseph S.

In: Learning and Memory, Vol. 5, No. 4-5, 1998, p. 391-403.

Research output: Contribution to journalArticle

Valentinuzzi, VS, Kolker, DE, Vitaterna, MH, Shimomura, K, Whiteley, A, Low-Zeddies, S, Turek, FW, Ferrari, EAM, Paylor, R & Takahashi, JS 1998, 'Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice', Learning and Memory, vol. 5, no. 4-5, pp. 391-403.
Valentinuzzi, Veronica S. ; Kolker, Daniel E. ; Vitaterna, Martha Hotz ; Shimomura, Kazuhiro ; Whiteley, Andrew ; Low-Zeddies, Sharon ; Turek, Fred W. ; Ferrari, Elenice A M ; Paylor, Richard ; Takahashi, Joseph S. / Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F2 mice. In: Learning and Memory. 1998 ; Vol. 5, No. 4-5. pp. 391-403.
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