Axonal transport: Imaging and modeling of a neuronal process

Sameer B. Shah, G. Yang, G. Danuser, L. S B Goldstein

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Owing to their unusual geometry and polarity, neurons face a tremendous transport challenge. In particular, the bi-directional movement of many cargoes between cell body and synapse that takes place within extremely long, narrow axons requires motor-driven active transport along polarized microtubules. We summarize some imaging and theoretical modeling strategies recently developed to better understand axonal transport and neuronal function. Our approaches are motivated by three questions: (1) Can we predict the response of a complex trafficking system to perturbations of various components, either alone, or in combination? (2) What is the relationship between in vitro measurements of single motor properties and the movement of motor-cargo complexes in vivo? (3) What key principles govern the operation of the neuronal transport system? We discuss the imaging of vesicular transport in Drosophila melanogaster larval axons, and the development of quantitative schemes to define transport function via the extraction of kinematic parameters from these images. The application of these schemes to images from wild-type larvae and larvae expressing mutations in specific transport proteins allows rigorous quantification of transport kinematics in functional and dysfunctional neurons. Finally, we present some strategies and results for the theoretical modeling of axonal transport, and discuss the integration of these results with experimental data.

Original languageEnglish (US)
Title of host publicationLecture Notes in Physics
Pages65-84
Number of pages20
Volume711
DOIs
StatePublished - 2007

Publication series

NameLecture Notes in Physics
Volume711
ISSN (Print)00758450

Fingerprint

larvae
axons
cargo
neurons
kinematics
Drosophila
synapses
mutations
complex systems
polarity
proteins
perturbation
geometry

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Shah, S. B., Yang, G., Danuser, G., & Goldstein, L. S. B. (2007). Axonal transport: Imaging and modeling of a neuronal process. In Lecture Notes in Physics (Vol. 711, pp. 65-84). (Lecture Notes in Physics; Vol. 711). https://doi.org/10.1007/3-540-49522-3_4

Axonal transport : Imaging and modeling of a neuronal process. / Shah, Sameer B.; Yang, G.; Danuser, G.; Goldstein, L. S B.

Lecture Notes in Physics. Vol. 711 2007. p. 65-84 (Lecture Notes in Physics; Vol. 711).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shah, SB, Yang, G, Danuser, G & Goldstein, LSB 2007, Axonal transport: Imaging and modeling of a neuronal process. in Lecture Notes in Physics. vol. 711, Lecture Notes in Physics, vol. 711, pp. 65-84. https://doi.org/10.1007/3-540-49522-3_4
Shah SB, Yang G, Danuser G, Goldstein LSB. Axonal transport: Imaging and modeling of a neuronal process. In Lecture Notes in Physics. Vol. 711. 2007. p. 65-84. (Lecture Notes in Physics). https://doi.org/10.1007/3-540-49522-3_4
Shah, Sameer B. ; Yang, G. ; Danuser, G. ; Goldstein, L. S B. / Axonal transport : Imaging and modeling of a neuronal process. Lecture Notes in Physics. Vol. 711 2007. pp. 65-84 (Lecture Notes in Physics).
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