A yeast artificial chromosome (YAC) contig from the C57BL/6 (H2b) mouse was created from the major histocompatibility complex (Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous > 1.5-Mb YAC contigs into a single contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 (H2(bc)) mouse, spanning approximately 600 kilobases, was also built from Znf173 (Afp, a gene for acid finger protein), through Tctex5 (t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs, which define the marker order as Cen-Znf173-Tctex5-Mog-D17Tu42-D17Mit232-H2-M3-D17Leh525-H2-M2-Tel. The gene order of Znf173-Tctex5-Mog-D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A.
- Major histocompatibility complex (MHC)
- Physical mapping
- Sequence tagged sites (STS)
ASJC Scopus subject areas