Barbiturate inhibition of lymphocyte function. Differing effects of various barbiturates used to induce coma

E. A. Neuwelt, K. Kikuchi, S. A. Hill, P. Lipsky, E. Frenkel

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The present studies evaluated the effect of phenobarbital, pentobarbital, and thiopental at concentrations comparable to those attained during therapeutic barbiturate-induced coma, on in vitro mitogen-induced lymphocyte activation. Lymphocytes from normal volunteers were incubated for 72 hours in culture medium containing mitogen (phytohemagglutinin) and a range of concentrations of the barbiturates (5 to 833 μg/ml). Three parameters of lymphocyte activation (mitogen-induced blast transformation, 3H-thymidine incorporation, and cell proliferation) were all suppressed by the barbiturates. The suppression was dose-dependent. The greatest suppression was caused by the short-acting barbiturate, thiopental. Lymphocyte responses were much less affected by the long-acting barbiturate, phenobarbital. The intermediate acting barbiturate, pentobarbital, was also intermediate in its ability to inhibit lymphocyte activation. The two- to threefold difference between the effects of thiopental and pentobarbital on lymphocyte function may have direct clinical relevance, since it is primarily these two agents that are employed to induce therapeutic 'barbiturate coma'. Since lymphocyte suppression appears to be much more marked in the presence of thiopental, these observations support a role for the other barbiturates in programs of induced coma.

Original languageEnglish (US)
Pages (from-to)254-259
Number of pages6
JournalJournal of Neurosurgery
Volume56
Issue number2
StatePublished - 1982

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Barbiturates
Coma
Thiopental
Lymphocyte Activation
Lymphocytes
Pentobarbital
Mitogens
Phenobarbital
Phytohemagglutinins
Thymidine
Culture Media
Healthy Volunteers
Cell Proliferation
barbituric acid
Therapeutics

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Barbiturate inhibition of lymphocyte function. Differing effects of various barbiturates used to induce coma. / Neuwelt, E. A.; Kikuchi, K.; Hill, S. A.; Lipsky, P.; Frenkel, E.

In: Journal of Neurosurgery, Vol. 56, No. 2, 1982, p. 254-259.

Research output: Contribution to journalArticle

Neuwelt, E. A. ; Kikuchi, K. ; Hill, S. A. ; Lipsky, P. ; Frenkel, E. / Barbiturate inhibition of lymphocyte function. Differing effects of various barbiturates used to induce coma. In: Journal of Neurosurgery. 1982 ; Vol. 56, No. 2. pp. 254-259.
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