Binding, internalization, and hydrolysis of low density lipoprotein in long term lymphoid cell lines from a normal subject and a patient with homozygous familial hypercholesterolemia

Y. K. Ho, M. S. Brown, H. J. Kayden, J. L. Goldstein

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Abstract

Long term established human lymphoid cells were shown to possess high affinity cell surface receptors for low density lipoprotein (LDL), the major cholesterol carrying protein in human plasma. Binding of LDL to these receptors was followed by internalization of the lipoprotein and hydrolysis of its protein and cholesteryl ester components. Cultured lymphocytes from a patient with the homozygous form of familial hypercholesterolemia lacked cell surface LDL receptors and therefore failed to take up and degrade the lipoprotein with high affinity. Cultured human lymphocytes should prove useful for further studies of: the relation between cholesterol metabolism and cellular function and the mechanism by which LDL binding at the cell surface leads to internalization of the lipoprotein.

Original languageEnglish (US)
Pages (from-to)444-455
Number of pages12
JournalJournal of Experimental Medicine
Volume144
Issue number2
StatePublished - 1976

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Hyperlipoproteinemia Type II
LDL Lipoproteins
Lipoproteins
Hydrolysis
LDL Receptors
Lymphocytes
Cell Line
Cholesterol Esters
Cell Surface Receptors
LDL Cholesterol
Proteins
Cholesterol

ASJC Scopus subject areas

  • Immunology

Cite this

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abstract = "Long term established human lymphoid cells were shown to possess high affinity cell surface receptors for low density lipoprotein (LDL), the major cholesterol carrying protein in human plasma. Binding of LDL to these receptors was followed by internalization of the lipoprotein and hydrolysis of its protein and cholesteryl ester components. Cultured lymphocytes from a patient with the homozygous form of familial hypercholesterolemia lacked cell surface LDL receptors and therefore failed to take up and degrade the lipoprotein with high affinity. Cultured human lymphocytes should prove useful for further studies of: the relation between cholesterol metabolism and cellular function and the mechanism by which LDL binding at the cell surface leads to internalization of the lipoprotein.",
author = "Ho, {Y. K.} and Brown, {M. S.} and Kayden, {H. J.} and Goldstein, {J. L.}",
year = "1976",
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T1 - Binding, internalization, and hydrolysis of low density lipoprotein in long term lymphoid cell lines from a normal subject and a patient with homozygous familial hypercholesterolemia

AU - Ho, Y. K.

AU - Brown, M. S.

AU - Kayden, H. J.

AU - Goldstein, J. L.

PY - 1976

Y1 - 1976

N2 - Long term established human lymphoid cells were shown to possess high affinity cell surface receptors for low density lipoprotein (LDL), the major cholesterol carrying protein in human plasma. Binding of LDL to these receptors was followed by internalization of the lipoprotein and hydrolysis of its protein and cholesteryl ester components. Cultured lymphocytes from a patient with the homozygous form of familial hypercholesterolemia lacked cell surface LDL receptors and therefore failed to take up and degrade the lipoprotein with high affinity. Cultured human lymphocytes should prove useful for further studies of: the relation between cholesterol metabolism and cellular function and the mechanism by which LDL binding at the cell surface leads to internalization of the lipoprotein.

AB - Long term established human lymphoid cells were shown to possess high affinity cell surface receptors for low density lipoprotein (LDL), the major cholesterol carrying protein in human plasma. Binding of LDL to these receptors was followed by internalization of the lipoprotein and hydrolysis of its protein and cholesteryl ester components. Cultured lymphocytes from a patient with the homozygous form of familial hypercholesterolemia lacked cell surface LDL receptors and therefore failed to take up and degrade the lipoprotein with high affinity. Cultured human lymphocytes should prove useful for further studies of: the relation between cholesterol metabolism and cellular function and the mechanism by which LDL binding at the cell surface leads to internalization of the lipoprotein.

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