Binding of antigen by immunocytes. II. Effect of specific Ig on antigen binding by MOPC 315 cells

J. C. Bystryn, G. W. Siskind, J. W. Uhr

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of specific immunoglobulin (Ig) on specific binding of antigen to cells has been studied in a model system consisting of murine myeloma cells (MOPC 315), MOPC 315 serum, and DNP conjugates. MOPC 315 serum, which has IgA specific for DNP, specifically inhibited the binding of DNP conjugates to these cells. Using this model it was found that cells have a marked advantage over free specific Ig in binding multivalent antigen molecules and retaining them in a bound state. Cells were able to specifically bind multivalent antigen in the presence of a large excess of free specific Ig. The kinetics of antigen binding to cells was slow, and prolongation of time of incubation increased the amount of specific binding. Both antihapten and anticarrier Ig augmented nonspecific binding of multivalent but not of univalent hapten to control cells. Furthermore, antihapten Ig at low concentration increased antigen binding to specific cells.

Original languageEnglish (US)
Pages (from-to)1227-1237
Number of pages11
JournalJournal of Experimental Medicine
Volume141
Issue number6
StatePublished - 1975

Fingerprint

Immunoglobulins
Antigens
Haptens
Serum
Immunoglobulin A

ASJC Scopus subject areas

  • Immunology

Cite this

Binding of antigen by immunocytes. II. Effect of specific Ig on antigen binding by MOPC 315 cells. / Bystryn, J. C.; Siskind, G. W.; Uhr, J. W.

In: Journal of Experimental Medicine, Vol. 141, No. 6, 1975, p. 1227-1237.

Research output: Contribution to journalArticle

Bystryn, J. C. ; Siskind, G. W. ; Uhr, J. W. / Binding of antigen by immunocytes. II. Effect of specific Ig on antigen binding by MOPC 315 cells. In: Journal of Experimental Medicine. 1975 ; Vol. 141, No. 6. pp. 1227-1237.
@article{2c7849104e914c9dbec4425a203e6552,
title = "Binding of antigen by immunocytes. II. Effect of specific Ig on antigen binding by MOPC 315 cells",
abstract = "The effect of specific immunoglobulin (Ig) on specific binding of antigen to cells has been studied in a model system consisting of murine myeloma cells (MOPC 315), MOPC 315 serum, and DNP conjugates. MOPC 315 serum, which has IgA specific for DNP, specifically inhibited the binding of DNP conjugates to these cells. Using this model it was found that cells have a marked advantage over free specific Ig in binding multivalent antigen molecules and retaining them in a bound state. Cells were able to specifically bind multivalent antigen in the presence of a large excess of free specific Ig. The kinetics of antigen binding to cells was slow, and prolongation of time of incubation increased the amount of specific binding. Both antihapten and anticarrier Ig augmented nonspecific binding of multivalent but not of univalent hapten to control cells. Furthermore, antihapten Ig at low concentration increased antigen binding to specific cells.",
author = "Bystryn, {J. C.} and Siskind, {G. W.} and Uhr, {J. W.}",
year = "1975",
language = "English (US)",
volume = "141",
pages = "1227--1237",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "6",

}

TY - JOUR

T1 - Binding of antigen by immunocytes. II. Effect of specific Ig on antigen binding by MOPC 315 cells

AU - Bystryn, J. C.

AU - Siskind, G. W.

AU - Uhr, J. W.

PY - 1975

Y1 - 1975

N2 - The effect of specific immunoglobulin (Ig) on specific binding of antigen to cells has been studied in a model system consisting of murine myeloma cells (MOPC 315), MOPC 315 serum, and DNP conjugates. MOPC 315 serum, which has IgA specific for DNP, specifically inhibited the binding of DNP conjugates to these cells. Using this model it was found that cells have a marked advantage over free specific Ig in binding multivalent antigen molecules and retaining them in a bound state. Cells were able to specifically bind multivalent antigen in the presence of a large excess of free specific Ig. The kinetics of antigen binding to cells was slow, and prolongation of time of incubation increased the amount of specific binding. Both antihapten and anticarrier Ig augmented nonspecific binding of multivalent but not of univalent hapten to control cells. Furthermore, antihapten Ig at low concentration increased antigen binding to specific cells.

AB - The effect of specific immunoglobulin (Ig) on specific binding of antigen to cells has been studied in a model system consisting of murine myeloma cells (MOPC 315), MOPC 315 serum, and DNP conjugates. MOPC 315 serum, which has IgA specific for DNP, specifically inhibited the binding of DNP conjugates to these cells. Using this model it was found that cells have a marked advantage over free specific Ig in binding multivalent antigen molecules and retaining them in a bound state. Cells were able to specifically bind multivalent antigen in the presence of a large excess of free specific Ig. The kinetics of antigen binding to cells was slow, and prolongation of time of incubation increased the amount of specific binding. Both antihapten and anticarrier Ig augmented nonspecific binding of multivalent but not of univalent hapten to control cells. Furthermore, antihapten Ig at low concentration increased antigen binding to specific cells.

UR - http://www.scopus.com/inward/record.url?scp=0016704117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016704117&partnerID=8YFLogxK

M3 - Article

VL - 141

SP - 1227

EP - 1237

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 6

ER -