@inbook{f07f1ea1b9f345af931c5634a08aea3c,
title = "Biochemical and functional assays of human cohesin-releasing factor Wapl",
abstract = "During the cell cycle, duplicated sister chromatids become physically connected during S phase through a process called sister-chromatid cohesion. Cohesion is terminated during the metaphase-to-anaphase transition to trigger sister-chromatid segregation. The establishment and dissolution of cohesion are highly regulated by the cohesin complex and its multitude of regulators. In particular, the cohesin regulator Wapl promotes the release of cohesin from chromosomes during both interphase and mitosis. Here, we describe in vitro protein binding assays between Wapl and a cohesin subcomplex, and cellular assays in human cells that probe the functions of Wapl in cohesin release.",
keywords = "Cohesin, Flow cytometry, In vitro binding assay, Metaphase spreads, Protein purification, Sisterchromatid cohesion, Wapl",
author = "Ge Zheng and Zhuqing Ouyang and Hongtao Yu",
note = "Funding Information: We thank Drs. Kodai Hara and Qianhui Qu for their input in the purification of SA2 and Wapl proteins. We thank all Yu lab members for helpful discussions and critical reading of the manuscript. H.Y. is an investigator with the Howard Hughes Medical Institute. Work in the Yu lab is supported by grants from the Welch Foundation (I-1441 to H.Y.), the Clayton Foundation, and Cancer Prevention and Research Institute of Texas (RP110465-P3 and RP120717-P2 to H.Y.).",
year = "2017",
doi = "10.1007/978-1-4939-6545-8_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "37--53",
booktitle = "Methods in Molecular Biology",
}