Biochemical and functional assays of human cohesin-releasing factor Wapl

Ge Zheng, Zhuqing Ouyang, Hongtao Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

During the cell cycle, duplicated sister chromatids become physically connected during S phase through a process called sister-chromatid cohesion. Cohesion is terminated during the metaphase-to-anaphase transition to trigger sister-chromatid segregation. The establishment and dissolution of cohesion are highly regulated by the cohesin complex and its multitude of regulators. In particular, the cohesin regulator Wapl promotes the release of cohesin from chromosomes during both interphase and mitosis. Here, we describe in vitro protein binding assays between Wapl and a cohesin subcomplex, and cellular assays in human cells that probe the functions of Wapl in cohesin release.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-53
Number of pages17
Volume1515
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1515
ISSN (Print)10643745

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Keywords

  • Cohesin
  • Flow cytometry
  • In vitro binding assay
  • Metaphase spreads
  • Protein purification
  • Sisterchromatid cohesion
  • Wapl

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zheng, G., Ouyang, Z., & Yu, H. (2017). Biochemical and functional assays of human cohesin-releasing factor Wapl. In Methods in Molecular Biology (Vol. 1515, pp. 37-53). (Methods in Molecular Biology; Vol. 1515). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6545-8_3