Biochemical characterization of Ia alloantigens in guinea pigs. I. Synthesis of Ia antigens by subsets enriched for B cells, T cells, or macrophages

C. R. Lyons, M. F. Lipscomb, S. F. Schlossman, T. F. Tucker, J. W. Uhr

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Abstract

Peritoneal exudate cells from immune guinea pigs consist primarily of T lymphocytes (PEL) and Mφ. After selection of PEL from animals immunized with ovalbumin by culture on specific antigen-pulsed syngeneic Mφ, recovered T cells (selected PEL) are 75 to 95% Ia + by both cytotoxicity and immunofluorescent analysis on the FACS using alloantisera to Ia from guinea pigs or mice. The subunit structure of the Ia molecules on T cells is similar to that of Ia molecules obtained from B cells, as determined by radiolabeling, immunoprecipitation, and analysis of gels. Incorporation of labeled amino acids into Ia by selected PEL was shown to be due to T cells because of the lack of effect of depletion of Ig + cells, the elimination of incorporation with a monoclonal anti-lymphocyte antibody, and the negligible increase in radioactive Ia after addition of large numbers of peritoneal exudate Mφ. Furthermore, when F 1 (2 x 3) PEL are selected on parental M∞, the selected PEL express both parental (2 and 13) Ia specificities, which suggests that the Ia molecules are not adsorbed by T cells after their release by Mφ. Evidence that synthesis by splenocytes is due to B cells was obtained by the results of deletion experiments with α Ig and C. Similarly, synthesis of Ia by populations of pulmonary alveolar Mφ, which contain >98% Mφ as judged by morphology, adherence, and latex ingestion, was demonstrated to be due to Mφ because of the lack of effect of removing T and B cells.

Original languageEnglish (US)
Pages (from-to)1879-1885
Number of pages7
JournalJournal of Immunology
Volume127
Issue number5
StatePublished - 1981

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B-Lymphocyte Subsets
Isoantigens
Histocompatibility Antigens Class II
Guinea Pigs
Macrophages
T-Lymphocytes
B-Lymphocytes
Exudates and Transudates
Latex
Ovalbumin
Immunoprecipitation
Anti-Idiotypic Antibodies
Eating
Gels
Monoclonal Antibodies
Lymphocytes
Antigens
Amino Acids
Lung
Population

ASJC Scopus subject areas

  • Immunology

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Biochemical characterization of Ia alloantigens in guinea pigs. I. Synthesis of Ia antigens by subsets enriched for B cells, T cells, or macrophages. / Lyons, C. R.; Lipscomb, M. F.; Schlossman, S. F.; Tucker, T. F.; Uhr, J. W.

In: Journal of Immunology, Vol. 127, No. 5, 1981, p. 1879-1885.

Research output: Contribution to journalArticle

Lyons, C. R. ; Lipscomb, M. F. ; Schlossman, S. F. ; Tucker, T. F. ; Uhr, J. W. / Biochemical characterization of Ia alloantigens in guinea pigs. I. Synthesis of Ia antigens by subsets enriched for B cells, T cells, or macrophages. In: Journal of Immunology. 1981 ; Vol. 127, No. 5. pp. 1879-1885.
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