Biochemical events associated with activation of smooth muscle contraction

W. C. Miller-Hance, J. R. Miller, J. N. Wells, J. T. Stull, K. E. Kamm

Research output: Contribution to journalArticle

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Abstract

Biochemical events associated with activation of smooth muscle contraction were studied in neurally stimulated bovine tracheal smooth muscle. A latency period of 500 ms preceded increases in isometric force and myosin light chain phosphorylation. However, stimulation resulted in the rapid hydrolysis of inositol phospholipids as demonstrated by increases in inositol phosphates by 500 ms. Inositol trisphosphate increased 2-fold with no significant change in inositol tetrakisphosphate. The apparent activation state of myosin light chain kinase was assessed indirectly through measurements of the fractional activation of a second calmodulin-dependent enzyme, cyclic nucleotide phosphodiesterase. The fractional activation of cyclic nucleotide phosphodiesterase increased after neural stimulation to a maximal extent by 500 ms and remained at this level for at least 4 s. The monophosphorylation of myosin light chain increased after 500 ms and reached a maximum value by 2 s. Diphosphorylation also occurred but to a much lesser extent. Fractional activation of cyclic nucleotide phosphodiesterase and myosin light chain phosphorylation both decreased after 10 min continuous stimulation, although the force response remained at a maximal level. These observations demonstrate that inositol trisphosphate formation and activation of cyclic nucleotide phosphodiesterase (and hence most likely myosin light chain kinase) by calmodulin precede myosin light chain phosphorylation and that these events are sufficiently rapid to mediate the contractile response of neurally stimulated tracheal smooth muscle.

Original languageEnglish (US)
Pages (from-to)13979-13982
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number28
StatePublished - 1988

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Myosin Light Chains
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Muscle Contraction
Smooth Muscle
Muscle
Inositol
Chemical activation
Myosin-Light-Chain Kinase
Phosphorylation
Calmodulin
Inositol Phosphates
Phosphatidylinositols
Hydrolysis
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biochemical events associated with activation of smooth muscle contraction. / Miller-Hance, W. C.; Miller, J. R.; Wells, J. N.; Stull, J. T.; Kamm, K. E.

In: Journal of Biological Chemistry, Vol. 263, No. 28, 1988, p. 13979-13982.

Research output: Contribution to journalArticle

Miller-Hance, WC, Miller, JR, Wells, JN, Stull, JT & Kamm, KE 1988, 'Biochemical events associated with activation of smooth muscle contraction', Journal of Biological Chemistry, vol. 263, no. 28, pp. 13979-13982.
Miller-Hance, W. C. ; Miller, J. R. ; Wells, J. N. ; Stull, J. T. ; Kamm, K. E. / Biochemical events associated with activation of smooth muscle contraction. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 28. pp. 13979-13982.
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