Biological properties of type C virus isolated from a human x mouse hybrid cell line

A. F. Gazdar, E. K. Russell, J. Minna

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The biological properties of the HMV 1 virus, spontaneously released from a human x C57BL/6 mouse hybrid cell line, were similar to those of RadLV, the prototype B tropic virus of C57BL/6 mice. Both viruses replicated on B type mouse cells and in the wild mouse cell line SC 1. The plaque forming abilities of the two viruses were relatively low, but gradually increased after passage in new host cells. Both viruses were neutralized by AKR antisera but not by FMR antisera. HMV 1 virus could rescue the defective sarcoma genome from S+H- mouse cells. The pseudotype sarcoma virus so produced was deficient in 'helper virus' activity. Newborn mice inoculated with HMV 1 virus remained tumor free over a 1 yr observation period.

Original languageEnglish (US)
Pages (from-to)688-692
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume149
Issue number3
StatePublished - 1975

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Hybrid Cells
Viruses
Cells
Cell Line
Inbred C57BL Mouse
Sarcoma
Immune Sera
Radiation Leukemia Virus
Helper Viruses
Cercopithecine Herpesvirus 1
Oncogenic Viruses
Tropics
Ferromagnetic resonance
Observation
Genome
Tumors
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "The biological properties of the HMV 1 virus, spontaneously released from a human x C57BL/6 mouse hybrid cell line, were similar to those of RadLV, the prototype B tropic virus of C57BL/6 mice. Both viruses replicated on B type mouse cells and in the wild mouse cell line SC 1. The plaque forming abilities of the two viruses were relatively low, but gradually increased after passage in new host cells. Both viruses were neutralized by AKR antisera but not by FMR antisera. HMV 1 virus could rescue the defective sarcoma genome from S+H- mouse cells. The pseudotype sarcoma virus so produced was deficient in 'helper virus' activity. Newborn mice inoculated with HMV 1 virus remained tumor free over a 1 yr observation period.",
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