Bioresorbable filaments enhance nerve regeneration

T. T. Ngo, A. Romero, K. D. Nelson, R. C. Eberhart, G. M. Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Although axonal regeneration does occur after injury in the peripheral nervous system (PNS), growth across a gap and functional restoration is as yet an unmet challenge. To improve axonal and functional nerve regeneration, we examined bioresorbable neural stents designed to organize cellular migration and direct axon growth across a lesion. Extruded and drawn (6:1) poly(L-lactide) (PLLA) (MW 200 kD) filaments were bundled, placed into silicone tubes (2 mm O.D., 1.5 mm I.D.) and used to bridge a 10-mm gap in the rat sciatic nerve. Empty silicone tubes served as controls. In a companion experiment, filament packing densities of 0% (0 filament), 3.75% (16), 7.5% (32), 15% (64), and 30% (128) with wet-spun PLLA filaments (MW 200 kD) were tested. After 10 weeks, animals were perfused and implants were prepared for histological analyses. Light microscopy and TEM both showed improved regeneration for extruded and wet-spun filaments versus controls, in terms of nerve cable formation and number of myelinated axons bridging the gap. In addition, the morphology of the regenerated nerve segment was similar to the multifascicular structure of uninjured nerve. Filament packing densities of 3.75-7.5% gave the best in improvement of nerve regeneration. We demonstrate that PLLA filament bundles enhance the extent and consistency of nerve regeneration across a gap. The results suggest that our PLLA filament bundles might be used to establish functional connectivity after nerve injury.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages373
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Silicones
Stents
Neurology
Restoration
Optical microscopy
Rats
Cables
Animals
Transmission electron microscopy
Experiments
Axons
poly(lactide)

ASJC Scopus subject areas

  • Bioengineering

Cite this

Ngo, T. T., Romero, A., Nelson, K. D., Eberhart, R. C., & Smith, G. M. (1999). Bioresorbable filaments enhance nerve regeneration. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 373). Piscataway, NJ, United States: IEEE.

Bioresorbable filaments enhance nerve regeneration. / Ngo, T. T.; Romero, A.; Nelson, K. D.; Eberhart, R. C.; Smith, G. M.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 373.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ngo, TT, Romero, A, Nelson, KD, Eberhart, RC & Smith, GM 1999, Bioresorbable filaments enhance nerve regeneration. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 373, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Ngo TT, Romero A, Nelson KD, Eberhart RC, Smith GM. Bioresorbable filaments enhance nerve regeneration. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 373
Ngo, T. T. ; Romero, A. ; Nelson, K. D. ; Eberhart, R. C. ; Smith, G. M. / Bioresorbable filaments enhance nerve regeneration. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 373
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