Body mass index-independent inflammation in omental adipose tissue associated with insulin resistance in morbid obesity

Olga T. Hardy, Richard A. Perugini, Sarah M. Nicoloro, Karen Gallagher-Dorval, Vishwajeet Puri, Juerg Straubhaar, Michael P. Czech

Research output: Contribution to journalArticle

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Abstract

Background: Obesity is a strong risk factor for resistance to insulin-mediated glucose disposal, a precursor of type 2 diabetes and other disorders. However, not all obese individuals are insulin resistant. We sought to identify the molecular pathways that might cause obesity-associated insulin resistance in humans by studying the morbidly obese who were insulin sensitive versus insulin resistant, thereby eliminating obesity as a variable. Methods: Combining gene expression profiling with computational approaches, we determined the global gene expression signatures of omental and subcutaneous adipose tissue samples obtained from similarly obese patients undergoing gastric bypass surgery. Results: Gene sets related to chemokine activity and chemokine receptor binding were identified as most highly expressed in the omental tissue from insulin-resistant compared with insulin-sensitive subjects, independent of the body mass index. These upregulated genes included chemokines (C-C motif) ligand 2, 3, 4, and 18 and interleukin-8/(CC-X motif) ligand 8 and were not differentially expressed in the subcutaneous adipose tissues between the 2 groups of subjects. Insulin resistance, but not the body mass index, was associated with increased macrophage infiltration in the omental adipose tissue, as was adipocyte size, in these morbidly obese subjects. Conclusion: Our findings have demonstrated that inflammation of the omental adipose tissue is strongly associated with insulin resistance in human obesity even in subjects with similar body mass index values.

Original languageEnglish (US)
Pages (from-to)60-67
Number of pages8
JournalSurgery for Obesity and Related Diseases
Volume7
Issue number1
DOIs
StatePublished - Jan 2011

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Morbid Obesity
Insulin Resistance
Adipose Tissue
Body Mass Index
Insulin
Inflammation
Obesity
Subcutaneous Fat
Chemokine CCL3
Gastric Bypass
Chemokine CCL2
Chemokine Receptors
Gene Expression Profiling
Interleukin-8
Transcriptome
Chemokines
Adipocytes
Type 2 Diabetes Mellitus
Genes
Macrophages

Keywords

  • Abdominal adipose tissue
  • Chemokines
  • Inflammation
  • Insulin resistance
  • Macrophages

ASJC Scopus subject areas

  • Surgery

Cite this

Body mass index-independent inflammation in omental adipose tissue associated with insulin resistance in morbid obesity. / Hardy, Olga T.; Perugini, Richard A.; Nicoloro, Sarah M.; Gallagher-Dorval, Karen; Puri, Vishwajeet; Straubhaar, Juerg; Czech, Michael P.

In: Surgery for Obesity and Related Diseases, Vol. 7, No. 1, 01.2011, p. 60-67.

Research output: Contribution to journalArticle

Hardy, Olga T. ; Perugini, Richard A. ; Nicoloro, Sarah M. ; Gallagher-Dorval, Karen ; Puri, Vishwajeet ; Straubhaar, Juerg ; Czech, Michael P. / Body mass index-independent inflammation in omental adipose tissue associated with insulin resistance in morbid obesity. In: Surgery for Obesity and Related Diseases. 2011 ; Vol. 7, No. 1. pp. 60-67.
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