Cacao polyphenols ameliorate autoimmune myocarditis in mice

Hirofumi Zempo, Jun Ichi Suzuki, Ryo Watanabe, Kouji Wakayama, Hidetoshi Kumagai, Yuichi Ikeda, Hiroshi Akazawa, Issei Komuro, Mitsuaki Isobe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Myocarditis is a clinically severe disease; however, no effective treatment has been established. The aim of this study was to determine whether cacao bean (Theobroma cacao) polyphenols ameliorate autoimmune myocarditis. We used an experimental autoimmune myocarditis (EAM) model in Balb/c mice. Mice with induced EAM were treated with a cacao polyphenol extract (CPE, n=12) or vehicle (n=12). On day 21, hearts were harvested and analyzed. Elevated heart weight to body weight and fibrotic area ratios as well as high cardiac cell infiltration were observed in the vehicle-treated EAM mice. However, these increases were significantly suppressed in the CPE-treated mice. Reverse transcriptase-PCR revealed that mRNA expressions of interleukin (Il)-1β, Il-6, E-selectin, vascular cell adhesion molecule-1 and collagen type 1 were lower in the CPE group compared with the vehicle group. The mRNA expressions of nicotinamide adenine dinucleotide phosphate-oxidase (Nox)2 and Nox4 were increased in the vehicle-treated EAM hearts, although CPE treatment did not significantly suppress the transcription levels. However, compared with vehicle treatment of EAM hearts, CPE treatment significantly suppressed hydrogen peroxide concentrations. Cardiac myeloperoxidase activity, the intensity of dihydroethidium staining and the phosphorylation of nuclear factor-κB p65 were also lower in the CPE group compared with the vehicle group. Our data suggest that CPE ameliorates EAM in mice. CPE is a promising dietary supplement to suppress cardiovascular inflammation and oxidative stress.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalHypertension Research
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Cacao
Myocarditis
Polyphenols
Messenger RNA
E-Selectin
Vascular Cell Adhesion Molecule-1
Dietary Supplements
Collagen Type I
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-1
NADP
Hydrogen Peroxide
Peroxidase
Interleukin-6
Oxidoreductases
Oxidative Stress
Body Weight
Phosphorylation
Staining and Labeling
Inflammation

Keywords

  • cacao polyphenols
  • inflammation
  • myocarditis
  • oxidative stress

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Zempo, H., Suzuki, J. I., Watanabe, R., Wakayama, K., Kumagai, H., Ikeda, Y., ... Isobe, M. (2016). Cacao polyphenols ameliorate autoimmune myocarditis in mice. Hypertension Research, 39(4), 203-209. https://doi.org/10.1038/hr.2015.136

Cacao polyphenols ameliorate autoimmune myocarditis in mice. / Zempo, Hirofumi; Suzuki, Jun Ichi; Watanabe, Ryo; Wakayama, Kouji; Kumagai, Hidetoshi; Ikeda, Yuichi; Akazawa, Hiroshi; Komuro, Issei; Isobe, Mitsuaki.

In: Hypertension Research, Vol. 39, No. 4, 01.04.2016, p. 203-209.

Research output: Contribution to journalArticle

Zempo, H, Suzuki, JI, Watanabe, R, Wakayama, K, Kumagai, H, Ikeda, Y, Akazawa, H, Komuro, I & Isobe, M 2016, 'Cacao polyphenols ameliorate autoimmune myocarditis in mice', Hypertension Research, vol. 39, no. 4, pp. 203-209. https://doi.org/10.1038/hr.2015.136
Zempo H, Suzuki JI, Watanabe R, Wakayama K, Kumagai H, Ikeda Y et al. Cacao polyphenols ameliorate autoimmune myocarditis in mice. Hypertension Research. 2016 Apr 1;39(4):203-209. https://doi.org/10.1038/hr.2015.136
Zempo, Hirofumi ; Suzuki, Jun Ichi ; Watanabe, Ryo ; Wakayama, Kouji ; Kumagai, Hidetoshi ; Ikeda, Yuichi ; Akazawa, Hiroshi ; Komuro, Issei ; Isobe, Mitsuaki. / Cacao polyphenols ameliorate autoimmune myocarditis in mice. In: Hypertension Research. 2016 ; Vol. 39, No. 4. pp. 203-209.
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