Calcitonin receptor-stimulating peptide-1 regulates ion transport and growth of renal epithelial cell line LLC-PK1

Kazumasa Hamano, Takeshi Katafuchi, Katsuro Kikumoto, Naoto Minamino

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Calcitonin receptor-stimulating peptide-1 (CRSP-1) is a peptide recently identified from porcine brain by monitoring the cAMP production through an endogenous calcitonin (CT) receptor in the renal epithelial cell line LLC-PK1. Here we investigated the effects of CRSP-1 on the ion transport and growth of LLC-PK1 cells. CRSP-1 inhibited the growth of LLC-PK1 cells with a higher potency than porcine CT. CRSP-1 enhanced the uptake of 22Na+ into LLC-PK1 cells more strongly than did CT and slightly reduced the 45Ca2+ uptake. The enhancement of the 22Na+ uptake was abolished by 5-(N-ethyl-N-isopropyl) amiloride, a strong Na+/H+ exchanger (NHE) inhibitor for NHE1, even at a concentration of 1 × 10 -8 M, although other ion transporter inhibitors did not affect the 22Na+ uptake. These results indicate that CRSP-1 enhances the 22Na+ uptake by the specific activation of NHE1. Taken together, CRSP-1 is considered to be a new regulator for the urinary ion excretion and renal epithelial cell growth.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number1
DOIs
StatePublished - Apr 29 2005

Keywords

  • 5-(N-Ethyl-N-isopropyl) amiloride
  • Calcitonin
  • Calcitonin receptor
  • Calcitonin receptor-stimulating peptide
  • LLC-PK cell
  • Na/H exchanger
  • cAMP
  • cAMP-dependent protein kinase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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