Calcium regulation of gelsolin and adseverin: A natural test of the helix latch hypothesis

Andreas Lueck, Helen L. Yin, David J. Kwiatkowski, Philip G. Allen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The gelsolin family of actin filament binding proteins have highly homologous structures. Gelsolin and adseverin, also known as scinderin, are the most similar members of this family, with adseverin lacking a C-terminal helix found in gelsolin. This helix has been postulated to serve as a calcium-sensitive latch, keeping gelsolin inactive. To test this hypothesis, we have analyzed the kinetics of severing by gelsolin, adseverin, and a gelsolin truncate which lacks the C-terminal latch. We find that the relationship between severing rate and calcium ion concentration differs between gelsolin and adseverin, and suggest that calcium controls one rate- limiting step in the activation of adseverin and two in the activation of gelsolin. In contrast, both proteins are activated equally by protons, and have identical severing kinetics at pHs below 6.3. The temperature sensitivity of severing by adseverin and gelsolin is remarkably different, with gelsolin increasing its severing rate 8-fold per 10 °C increase in temperature and adseverin increasing its rate only 2-fold per 10 °C increase in temperature. Analysis of the gelsolin construct lacking the C-terminal helix demonstrates that this helix is responsible for the regulatory differences between gelsolin and adseverin. These results support the C- terminal latch hypothesis for the calcium ion activation of gelsolin.

Original languageEnglish (US)
Pages (from-to)5274-5279
Number of pages6
JournalBiochemistry
Volume39
Issue number18
DOIs
StatePublished - May 9 2000

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Gelsolin
Calcium
Chemical activation
scinderin
Temperature
Ions
Microfilament Proteins
Kinetics
Actin Cytoskeleton

ASJC Scopus subject areas

  • Biochemistry

Cite this

Calcium regulation of gelsolin and adseverin : A natural test of the helix latch hypothesis. / Lueck, Andreas; Yin, Helen L.; Kwiatkowski, David J.; Allen, Philip G.

In: Biochemistry, Vol. 39, No. 18, 09.05.2000, p. 5274-5279.

Research output: Contribution to journalArticle

Lueck, Andreas ; Yin, Helen L. ; Kwiatkowski, David J. ; Allen, Philip G. / Calcium regulation of gelsolin and adseverin : A natural test of the helix latch hypothesis. In: Biochemistry. 2000 ; Vol. 39, No. 18. pp. 5274-5279.
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