Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle

T. A. Zainal, T. D. Oberley, D. B. Allison, L. I. Szweda, R. Weindruch

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

In laboratory rodents, caloric restriction (CR) retards several age-dependent physiological and biochemical changes in skeletal muscle, including increased steady-state levels of oxidative damage to lipids, DNA, and proteins. We used immunogold electron microscopic (EM) techniques with antibodies raised against 4-hydroxy-2-nonenal (HNE) -modified proteins dinitrophenol, and nitrotyrosine to quantify and localize the age-dependent accrual of oxidative damage in rhesus monkey vastus lateralis skeletal muscle. Using immunogold EM analysis of muscle from rhesus monkeys ranging in age from 2 to 34 years old, a fourfold maximal increase in levels of HNE-modified proteins was observed. Likewise, carbonyl levels increased ~ twofold with aging. Comparing 17- to 23-year-old normally fed to age-matched monkeys subjected to CR for 10 years, levels of HNE-modified proteins, carbonyls, and nitrotyrosine in skeletal muscle from the CR group were significantly less than control group values. Oxidative damage largely localized to myofibrils, with lesser labeling in other subcellular compartments. Accumulation of lipid peroxidation-derived aldehydes, such as malondialdehyde and 4-hydroxy-2-alkenals, and protein carbonyls were measured biochemically and confirmed the morphological data. Our study is the first to quantify morphologically and localize the age-dependent accrual of oxidative damage in mammalian skeletal muscle and to demonstrate that oxidative damage in primates is lowered by CR.

Original languageEnglish (US)
Pages (from-to)1825-1836
Number of pages12
JournalFASEB Journal
Volume14
Issue number12
StatePublished - Sep 23 2000

Fingerprint

Caloric Restriction
Macaca mulatta
Muscle
skeletal muscle
Skeletal Muscle
Proteins
proteins
electrons
Electrons
Dinitrophenols
Lipids
Myofibrils
myofibrils
Quadriceps Muscle
Malondialdehyde
Aldehydes
Labeling
aldehydes
malondialdehyde
Primates

Keywords

  • Aging
  • Free radicals
  • Immunogold
  • Lipid peroxidation
  • Reactive oxygen species
  • Sarcopenia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Zainal, T. A., Oberley, T. D., Allison, D. B., Szweda, L. I., & Weindruch, R. (2000). Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle. FASEB Journal, 14(12), 1825-1836.

Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle. / Zainal, T. A.; Oberley, T. D.; Allison, D. B.; Szweda, L. I.; Weindruch, R.

In: FASEB Journal, Vol. 14, No. 12, 23.09.2000, p. 1825-1836.

Research output: Contribution to journalArticle

Zainal, TA, Oberley, TD, Allison, DB, Szweda, LI & Weindruch, R 2000, 'Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle', FASEB Journal, vol. 14, no. 12, pp. 1825-1836.
Zainal TA, Oberley TD, Allison DB, Szweda LI, Weindruch R. Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle. FASEB Journal. 2000 Sep 23;14(12):1825-1836.
Zainal, T. A. ; Oberley, T. D. ; Allison, D. B. ; Szweda, L. I. ; Weindruch, R. / Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle. In: FASEB Journal. 2000 ; Vol. 14, No. 12. pp. 1825-1836.
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