Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations

Sunghoon Kim, Weizhong Zeng, Shane Bernard, Jun Liao, Muthusubramanian Venkateshwaran, Jean Michel Ane, Youxing Jiang

Research output: Contribution to journalArticle

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Abstract

A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca2+ spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca2+ channel whose activation requires cytosolic/nucleosolic Ca2+, contrary to the previous suggestion of it being a K+ channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K+) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca2+-gated K+ (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca2+ sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca2+ binding sites to the physiological function of CASTOR as well as DMI1.

Original languageEnglish (US)
Article number3703
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

regulators
Binding Sites
infectious diseases
Large-Conductance Calcium-Activated Potassium Channels
Symbiosis
rings
Infection
Nuclear Family
Ion Channels
symbiosis
Fabaceae
Assays
spiking
Chemical activation
microorganisms
Ions
Ligands
suggestion
activation
ligands

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations. / Kim, Sunghoon; Zeng, Weizhong; Bernard, Shane; Liao, Jun; Venkateshwaran, Muthusubramanian; Ane, Jean Michel; Jiang, Youxing.

In: Nature communications, Vol. 10, No. 1, 3703, 01.12.2019.

Research output: Contribution to journalArticle

Kim, Sunghoon ; Zeng, Weizhong ; Bernard, Shane ; Liao, Jun ; Venkateshwaran, Muthusubramanian ; Ane, Jean Michel ; Jiang, Youxing. / Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations. In: Nature communications. 2019 ; Vol. 10, No. 1.
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