CCDC11 is a novel centriolar satellite protein essential for ciliogenesis and establishment of left-right asymmetry

Erica Silva, Ewelina Betleja, Emily John, Philip Spear, James J. Moresco, Siwei Zhang, John R. Yates, Brian J. Mitchell, Moe R. Mahjoub

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The establishment of left-right (L-R) asymmetry in vertebrates is dependent on the sensory and motile functions of cilia during embryogenesis. Mutations in CCDC11 disrupt L-R asymmetry and cause congenital heart disease in humans, yet the molecular and cellular functions of the protein remain unknown. Here we demonstrate that Ccdc11 is a novel component of centriolar satellites-cytoplasmic granules that serve as recruitment sites for proteins destined for the centrosome and cilium. Ccdc11 interacts with core components of satellites, and its loss disrupts the subcellular organization of satellite proteins and perturbs primary cilium assembly. Ccdc11 colocalizes with satellite proteins in human multiciliated tracheal epithelia, and its loss inhibits motile ciliogenesis. Similarly, depletion of CCDC11 in Xenopus embryos causes defective assembly and motility of cilia in multiciliated epidermal cells. To determine the role of CCDC11 during vertebrate development, we generated mutant alleles in zebrafish. Loss of CCDC11 leads to defective ciliogenesis in the pronephros and within the Kupffer's vesicle and results in aberrant L-R axis determination. Our results highlight a critical role for Ccdc11 in the assembly and function of motile cilia and implicate centriolar satellite-associated proteins as a new class of proteins in the pathology of L-R patterning and congenital heart disease.

Original languageEnglish (US)
Pages (from-to)48-63
Number of pages16
JournalMolecular biology of the cell
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Cilia
Proteins
Vertebrates
Heart Diseases
Pronephros
Cytoplasmic Granules
Centrosome
Zebrafish
Xenopus
Embryonic Development
Embryonic Structures
Epithelium
Alleles
Pathology
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

CCDC11 is a novel centriolar satellite protein essential for ciliogenesis and establishment of left-right asymmetry. / Silva, Erica; Betleja, Ewelina; John, Emily; Spear, Philip; Moresco, James J.; Zhang, Siwei; Yates, John R.; Mitchell, Brian J.; Mahjoub, Moe R.

In: Molecular biology of the cell, Vol. 27, No. 1, 01.01.2016, p. 48-63.

Research output: Contribution to journalArticle

Silva, Erica ; Betleja, Ewelina ; John, Emily ; Spear, Philip ; Moresco, James J. ; Zhang, Siwei ; Yates, John R. ; Mitchell, Brian J. ; Mahjoub, Moe R. / CCDC11 is a novel centriolar satellite protein essential for ciliogenesis and establishment of left-right asymmetry. In: Molecular biology of the cell. 2016 ; Vol. 27, No. 1. pp. 48-63.
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