C/EBPα regulates SIRT1 expression during adipogenesis

Qihuang Jin, Fang Zhang, Tingting Yan, Zhen Liu, Chunxi Wang, Xinjian Ge, Qiwei Zhai

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

SIRT1 plays an important role in adipogenesis, but how SIRT1 is regulated in adipogenesis is largely unknown. In this study, we show that both SIRT1 protein and mRNA levels were increased along with CCAAT/enhancer-binding protein α (C/EBPα) during adipocyte differentiation. C/EBPα, but not C/EBPαp30, activated SIRT1 promoter in both HeLa cells and 3T3-L1 preadipocytes. Furthermore, C/EBPα upregulated SIRT1 mRNA and protein levels in HeLa cells and increased SIRT1 expression in a p53-independent manner in Soas2 cells. In preadipocytes, ectopic expression of C/EBPα upregulated SIRT1 protein level and knockdown of C/EBPα led to the decrease of SIRT1 protein level. Moreover, by promoter deletion analysis, gel shift assay and chromatin immunoprecipitation, we found that C/EBPα bound to the SIRT1 promoter at a consensus C/EBPα binding site. These data demonstrate that C/EBPα regulates SIRT1 expression during adipogenesis by directly binding to the SIRT1 promoter.

Original languageEnglish (US)
Pages (from-to)470-479
Number of pages10
JournalCell Research
Volume20
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

CCAAT-Enhancer-Binding Proteins
Adipogenesis
HeLa Cells
Proteins
Messenger RNA
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
Protein C
Adipocytes
Protein Binding
Binding Sites

Keywords

  • Adipogenesis
  • C/EBPα
  • Obesity
  • SIRT1
  • Transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Jin, Q., Zhang, F., Yan, T., Liu, Z., Wang, C., Ge, X., & Zhai, Q. (2010). C/EBPα regulates SIRT1 expression during adipogenesis. Cell Research, 20(4), 470-479. https://doi.org/10.1038/cr.2010.24

C/EBPα regulates SIRT1 expression during adipogenesis. / Jin, Qihuang; Zhang, Fang; Yan, Tingting; Liu, Zhen; Wang, Chunxi; Ge, Xinjian; Zhai, Qiwei.

In: Cell Research, Vol. 20, No. 4, 01.04.2010, p. 470-479.

Research output: Contribution to journalArticle

Jin, Q, Zhang, F, Yan, T, Liu, Z, Wang, C, Ge, X & Zhai, Q 2010, 'C/EBPα regulates SIRT1 expression during adipogenesis', Cell Research, vol. 20, no. 4, pp. 470-479. https://doi.org/10.1038/cr.2010.24
Jin Q, Zhang F, Yan T, Liu Z, Wang C, Ge X et al. C/EBPα regulates SIRT1 expression during adipogenesis. Cell Research. 2010 Apr 1;20(4):470-479. https://doi.org/10.1038/cr.2010.24
Jin, Qihuang ; Zhang, Fang ; Yan, Tingting ; Liu, Zhen ; Wang, Chunxi ; Ge, Xinjian ; Zhai, Qiwei. / C/EBPα regulates SIRT1 expression during adipogenesis. In: Cell Research. 2010 ; Vol. 20, No. 4. pp. 470-479.
@article{4f7d7c3a212841fd83393abfdcf023a4,
title = "C/EBPα regulates SIRT1 expression during adipogenesis",
abstract = "SIRT1 plays an important role in adipogenesis, but how SIRT1 is regulated in adipogenesis is largely unknown. In this study, we show that both SIRT1 protein and mRNA levels were increased along with CCAAT/enhancer-binding protein α (C/EBPα) during adipocyte differentiation. C/EBPα, but not C/EBPαp30, activated SIRT1 promoter in both HeLa cells and 3T3-L1 preadipocytes. Furthermore, C/EBPα upregulated SIRT1 mRNA and protein levels in HeLa cells and increased SIRT1 expression in a p53-independent manner in Soas2 cells. In preadipocytes, ectopic expression of C/EBPα upregulated SIRT1 protein level and knockdown of C/EBPα led to the decrease of SIRT1 protein level. Moreover, by promoter deletion analysis, gel shift assay and chromatin immunoprecipitation, we found that C/EBPα bound to the SIRT1 promoter at a consensus C/EBPα binding site. These data demonstrate that C/EBPα regulates SIRT1 expression during adipogenesis by directly binding to the SIRT1 promoter.",
keywords = "Adipogenesis, C/EBPα, Obesity, SIRT1, Transcriptional regulation",
author = "Qihuang Jin and Fang Zhang and Tingting Yan and Zhen Liu and Chunxi Wang and Xinjian Ge and Qiwei Zhai",
year = "2010",
month = "4",
day = "1",
doi = "10.1038/cr.2010.24",
language = "English (US)",
volume = "20",
pages = "470--479",
journal = "Cell Research",
issn = "1001-0602",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - C/EBPα regulates SIRT1 expression during adipogenesis

AU - Jin, Qihuang

AU - Zhang, Fang

AU - Yan, Tingting

AU - Liu, Zhen

AU - Wang, Chunxi

AU - Ge, Xinjian

AU - Zhai, Qiwei

PY - 2010/4/1

Y1 - 2010/4/1

N2 - SIRT1 plays an important role in adipogenesis, but how SIRT1 is regulated in adipogenesis is largely unknown. In this study, we show that both SIRT1 protein and mRNA levels were increased along with CCAAT/enhancer-binding protein α (C/EBPα) during adipocyte differentiation. C/EBPα, but not C/EBPαp30, activated SIRT1 promoter in both HeLa cells and 3T3-L1 preadipocytes. Furthermore, C/EBPα upregulated SIRT1 mRNA and protein levels in HeLa cells and increased SIRT1 expression in a p53-independent manner in Soas2 cells. In preadipocytes, ectopic expression of C/EBPα upregulated SIRT1 protein level and knockdown of C/EBPα led to the decrease of SIRT1 protein level. Moreover, by promoter deletion analysis, gel shift assay and chromatin immunoprecipitation, we found that C/EBPα bound to the SIRT1 promoter at a consensus C/EBPα binding site. These data demonstrate that C/EBPα regulates SIRT1 expression during adipogenesis by directly binding to the SIRT1 promoter.

AB - SIRT1 plays an important role in adipogenesis, but how SIRT1 is regulated in adipogenesis is largely unknown. In this study, we show that both SIRT1 protein and mRNA levels were increased along with CCAAT/enhancer-binding protein α (C/EBPα) during adipocyte differentiation. C/EBPα, but not C/EBPαp30, activated SIRT1 promoter in both HeLa cells and 3T3-L1 preadipocytes. Furthermore, C/EBPα upregulated SIRT1 mRNA and protein levels in HeLa cells and increased SIRT1 expression in a p53-independent manner in Soas2 cells. In preadipocytes, ectopic expression of C/EBPα upregulated SIRT1 protein level and knockdown of C/EBPα led to the decrease of SIRT1 protein level. Moreover, by promoter deletion analysis, gel shift assay and chromatin immunoprecipitation, we found that C/EBPα bound to the SIRT1 promoter at a consensus C/EBPα binding site. These data demonstrate that C/EBPα regulates SIRT1 expression during adipogenesis by directly binding to the SIRT1 promoter.

KW - Adipogenesis

KW - C/EBPα

KW - Obesity

KW - SIRT1

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=77950538806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950538806&partnerID=8YFLogxK

U2 - 10.1038/cr.2010.24

DO - 10.1038/cr.2010.24

M3 - Article

C2 - 20157332

AN - SCOPUS:77950538806

VL - 20

SP - 470

EP - 479

JO - Cell Research

JF - Cell Research

SN - 1001-0602

IS - 4

ER -