C/EBP transcription factors mediate epicardial activation during heart development and injury

Guo N. Huang, Jeffrey E. Thatcher, John McAnally, Yongli Kong, Xiaoxia Qi, Wei Tan, J. Michael DiMaio, James F Amatruda, Robert D. Gerard, Joseph A Hill, Rhonda S Bassel-Duby, Eric N Olson

Research output: Contribution to journalArticlepeer-review

132 Scopus citations

Abstract

The epicardium encapsulates the heart and functions as a source of multipotent progenitor cells and paracrine factors essential for cardiac development and repair. Injury of the adult heart results in reactivation of a developmental gene program in the epicardium, but the transcriptional basis of epicardial gene expression has not been delineated. We established a mouse embryonic heart organ culture and gene expression system that facilitated the identification of epicardial enhancers activated during heart development and injury. Epicardial activation of these enhancers depends on a combinatorial transcriptional code centered on CCAAT/enhancer binding protein (C/EBP) transcription factors. Disruption of C/EBP signaling in the adult epicardium reduced injury-induced neutrophil infiltration and improved cardiac function. These findings reveal a transcriptional basis for epicardial activation and heart injury, providing a platform for enhancing cardiac regeneration.

Original languageEnglish (US)
Pages (from-to)1599-1603
Number of pages5
JournalScience
Volume338
Issue number6114
DOIs
StatePublished - Dec 21 2012

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'C/EBP transcription factors mediate epicardial activation during heart development and injury'. Together they form a unique fingerprint.

Cite this