Cell-mediated cytotoxicity against HLA-D-region products expressed in monocytes and B lymphocytes - I. Blocking by unlabelled cells and inhibition by antisera

Conleth F. Feighery, Peter Stastny

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Cytotoxic effector cells against HLA-D-region products were generated in cultures with HLA-A- and B-matched, HLA-D-mismatched stimulating cells. Monocytes from unrelated donors sharing HLA-D/DR antigens with the primary stimulator were used as target cells. Lysis of target cells was inhibited by addition of unlabelled monocytes having the same HLA-D/DR antigens. Inhibition of cytotoxicity was also observed with unlabelled B cells, but T lymphocytes had little effect. The distribution of the target antigens, therefore, fits the known distribution of the products of HLA-D. In other experiments, a human alloantiserum specific for HLA-DRw3 was found to inhibit cellular cytotoxicity specific for HLA-D/DRw3. Lysis by HLA-D/DRw3-specific effector cells was not inhibited by sera against HLA-DRw2 or DRw7 or by antibodies against HLA-B8 using HLA-B8 positive, DRw3 positive target cells. A xenogeneic serum against human Ia antigens, produced in rabbits, blocked cytotoxicity directed at DRw2, DRw3 and DRw4. These results suggest that cell-mediated cyctotoxicity was directed against HLA-D/DR or very closely associated determinants.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalImmunogenetics
Volume10
Issue number1-4
DOIs
StatePublished - Feb 1 1980

ASJC Scopus subject areas

  • Immunology
  • Genetics

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