Cell surface immunoglobulin: XX. Antibody responsiveness of subpopulations of b lymphocytes bearing different isotypes*

D. Yuan, E. S. Vitetta, J. R. Kettman

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Murine spleen cells were depleted of specific B-cell subpopulations bearing different immunoglobulin isotypes by means of complement-mediated cytolysis after treatment with antisera specific for µ- and γ-chains. The functional effect of this depletion was measured by assaying both the primary and secondary plaque-forming cell responses of the residual cells after transfer to carrierprimed lethally irradiated hosts. The results suggest that cells bearing IgM are the progenitors of plaque-forming cells in the primary response and cells bearing IgG are the major progenitors of IgG plaque-forming cells in the secondary response. The quantity of IgM on progenitors of secondary IgM plaque-forming cells decreases markedly as the interval between primary immunization and antigenic challenge increases. Long-term memory cells for the secondary IgM response bear small amounts of both IgM and IgG.

Original languageEnglish (US)
Pages (from-to)1421-1435
Number of pages15
JournalJournal of Experimental Medicine
Volume145
Issue number6
DOIs
StatePublished - Jun 1 1977

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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