Cell surface immunoglobulin. XX. Antibody responsiveness of subpopulations of B lymphocytes bearing different isotypes

D. Yuan, E. S. Vitetta, J. R. Kettman

Research output: Contribution to journalArticle

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Abstract

Murine spleen cells were depleted of specific B-cell subpopulations bearing different immunoglobulin isotypes by means of complement-mediated cytolysis after treatment with antisera specific for μ- and γ-chains. The functional effect of this depletion was measured by assaying both the primary and secondary plaque forming cell responses of the residual cells after transfer to carrier-primed lethally irradiated hosts. The results suggest that cells bearing IgM are the progenitors of plaque-forming cells in the primary response and cells bearing IgG are the major progenitors of IgG plaque-forming cells in the secondary response. The quantity of IgM on progenitors of secondary IgM plaque-forming cells decreases markedly as the interval between primary immunization and antigenic challenge increases. Long term memory cells for the secondary IgM response bear small amounts of both IgM and IgG.

Original languageEnglish (US)
Pages (from-to)1421-1435
Number of pages15
JournalJournal of Experimental Medicine
Volume145
Issue number6
StatePublished - 1977

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B-Cell Antigen Receptors
B-Lymphocytes
Antibodies
Immunoglobulin M
Immunoglobulin G
Long-Term Memory
Immunoglobulin Isotypes
Immune Sera
Immunization
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Cell surface immunoglobulin. XX. Antibody responsiveness of subpopulations of B lymphocytes bearing different isotypes. / Yuan, D.; Vitetta, E. S.; Kettman, J. R.

In: Journal of Experimental Medicine, Vol. 145, No. 6, 1977, p. 1421-1435.

Research output: Contribution to journalArticle

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