Abstract
Murine spleen cells were depleted of specific B-cell subpopulations bearing different immunoglobulin isotypes by means of complement-mediated cytolysis after treatment with antisera specific for µ- and γ-chains. The functional effect of this depletion was measured by assaying both the primary and secondary plaque-forming cell responses of the residual cells after transfer to carrierprimed lethally irradiated hosts. The results suggest that cells bearing IgM are the progenitors of plaque-forming cells in the primary response and cells bearing IgG are the major progenitors of IgG plaque-forming cells in the secondary response. The quantity of IgM on progenitors of secondary IgM plaque-forming cells decreases markedly as the interval between primary immunization and antigenic challenge increases. Long-term memory cells for the secondary IgM response bear small amounts of both IgM and IgG.
Original language | English (US) |
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Pages (from-to) | 1421-1435 |
Number of pages | 15 |
Journal | Journal of Experimental Medicine |
Volume | 145 |
Issue number | 6 |
DOIs | |
State | Published - Jun 1 1977 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology