Cell transplantation from limb allografts

Peter E.M. Butler, W. P.Andrew Lee, C. Derek Sims, Mark A. Randolph, Charles A. Vacanti, Michael J. Yaremchuk

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A murine model of skeletal tissue transplantation was developed to study the allograft rejection process in mice for limb allograft transplantation. Muscle, bone, and skin have been shown to be strong antigenic stimuli in vascularized allograft models, and cells from these sources were used for transplantation. Using enzymatic digestion, keratinocytes, myocytes, and osteocytes were harvested from B10.A mice tissues, dissociated into single cells, and then grown in culture for 14 to 21 days. Each cell type was marked with an intracellular fluorescent marker before transplantation of the cells into pockets in the rectus abdominis muscle of a syngenic host. All cell types remained viable and were detectable 2 weeks following transplantation when examined histologically and observed under a fluorescent microscope. Transplanted osteocytes were found to produce bone 8 weeks following transplantation. These results demonstrate that individual cells transplanted into muscle pockets survive and have the ability to produce extracellular matrix in this mouse model of skeletal tissue transplantation. Use of this model will allow transplantation of the cellular components comprising limb allografts to study the relative antigenicities and the rejection of the separate cells with the advanced immunologic techniques available for mice. A better understanding of immunologic responses to these individual tissue components may enable specific donor tissue or host immune modification to achieve skeletal tissue transplantation without immunosuppression. These findings are particularly valuable to the field of tissue engineering where allogeneic cells may be used in cell/polymer constructs for reconstructive procedures.

Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalPlastic and reconstructive surgery
Volume102
Issue number1
DOIs
StatePublished - Jul 1 1998
Externally publishedYes

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Cell Transplantation
Allografts
Extremities
Tissue Transplantation
Transplantation
Osteocytes
Muscles
Immunologic Techniques
Bone and Bones
Rectus Abdominis
Tissue Engineering
Keratinocytes
Immunosuppression
Muscle Cells
Extracellular Matrix
Digestion
Polymers
Tissue Donors
Skin

ASJC Scopus subject areas

  • Surgery

Cite this

Butler, P. E. M., Lee, W. P. A., Sims, C. D., Randolph, M. A., Vacanti, C. A., & Yaremchuk, M. J. (1998). Cell transplantation from limb allografts. Plastic and reconstructive surgery, 102(1), 161-170. https://doi.org/10.1097/00006534-199807000-00025

Cell transplantation from limb allografts. / Butler, Peter E.M.; Lee, W. P.Andrew; Sims, C. Derek; Randolph, Mark A.; Vacanti, Charles A.; Yaremchuk, Michael J.

In: Plastic and reconstructive surgery, Vol. 102, No. 1, 01.07.1998, p. 161-170.

Research output: Contribution to journalArticle

Butler, PEM, Lee, WPA, Sims, CD, Randolph, MA, Vacanti, CA & Yaremchuk, MJ 1998, 'Cell transplantation from limb allografts', Plastic and reconstructive surgery, vol. 102, no. 1, pp. 161-170. https://doi.org/10.1097/00006534-199807000-00025
Butler PEM, Lee WPA, Sims CD, Randolph MA, Vacanti CA, Yaremchuk MJ. Cell transplantation from limb allografts. Plastic and reconstructive surgery. 1998 Jul 1;102(1):161-170. https://doi.org/10.1097/00006534-199807000-00025
Butler, Peter E.M. ; Lee, W. P.Andrew ; Sims, C. Derek ; Randolph, Mark A. ; Vacanti, Charles A. ; Yaremchuk, Michael J. / Cell transplantation from limb allografts. In: Plastic and reconstructive surgery. 1998 ; Vol. 102, No. 1. pp. 161-170.
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