Changes in interfilament spacing mimic the effects of myosin regulatory light chain phosphorylation in rabbit psoas fibers

Zhaohui Yang, James T. Stull, Rhea J C Levine, H. Lee Sweeney

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The modulatory effect of myosin regulatory light chain phosphorylation in mammalian skeletal muscle, first documented as posttetanic potentiation of twitch tension, was subsequently shown to enhance the expression and development of tension at submaximal levels of activating calcium. Structural analyses demonstrated that thick filaments with phosphorylated myosin regulatory light chains appeared disordered: they lost the near-helical, periodic arrangement of myosin head characteristic of the relaxed state. We suggested that disordered heads may be more mobile than ordered heads and are likely to spend more time close to their binding sites on thin filaments. In this study we determined that the physiological effects of phosphorylation could be mimicked by decreasing the lattice spacing between the thick and the thin filaments, either by osmotic compression with dextran or by increasing the sarcomere length of permeabilized rabbit psoas fibers. Phosphorylation of regulatory light chains by incubation of permeabilized fibers with myosin light chain kinase and calmodulin, followed by low levels of activating calcium, potentiated tension development at resting or lower sarcomere lengths in the absence of dextran but had no additional effect on tension potentiation or development in fibers with decreased lattice spacing due to either osmotic compression or increased sarcomere length.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalJournal of Structural Biology
Volume122
Issue number1-2
DOIs
StatePublished - 1998

Keywords

  • Helical arrangement
  • Interfilament spacing
  • Myosin heads
  • Myosin regulatory light chain
  • Osmotic compression
  • Phosphorylation
  • Sarcomere length

ASJC Scopus subject areas

  • Structural Biology

Fingerprint Dive into the research topics of 'Changes in interfilament spacing mimic the effects of myosin regulatory light chain phosphorylation in rabbit psoas fibers'. Together they form a unique fingerprint.

  • Cite this