Characterization of a monoclonal antibody reactive with a subset of human breast tumors

D. Yuan, F. J. Hendler, E. S. Vitetta

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Fusion of spleen cells, from mice immunized against the human hormone-dependent breast cancer cell line ZR-75-1, with murine myeloma cells resulted in the establishment of a hybridoma cell line, HY59-H10 (H59). The purified monoclonal IgM produced by the hybridoma reacts with the most differentiated human breast tumor cell lines but not with cells derived from normal breast secretions or with numerous other malignant cell lines. Of 106 biopsy specimens examined, H59 bound to 54% of malignant breast specimens, to 83% of fibroadenomas, and to 82% of specimens containing fibrocystic disease. Binding could not be detected in uninvolved breast tissue.

Original languageEnglish (US)
Pages (from-to)719-728
Number of pages10
JournalJournal of the National Cancer Institute
Volume68
Issue number5
StatePublished - 1982

Fingerprint

Breast
Monoclonal Antibodies
Hybridomas
Breast Neoplasms
Cell Line
Fibroadenoma
Cell Fusion
Tumor Cell Line
Immunoglobulin M
Spleen
Hormones
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Characterization of a monoclonal antibody reactive with a subset of human breast tumors. / Yuan, D.; Hendler, F. J.; Vitetta, E. S.

In: Journal of the National Cancer Institute, Vol. 68, No. 5, 1982, p. 719-728.

Research output: Contribution to journalArticle

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