Characterization of cell populations used for studies of antibodies to I region products

M. E. Moraes, S. Sittler, P. Stastny

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Mononuclear cells from peripheral blood contained at least four different cell types: monocytes, T lymphocytes, B lymphocytes, and lymphocytes with Fc receptors. Monocytes represented 41% of B cell enriched preparations and gave rise to extra reactions due to monocyte antibodies in alloimmune sera. Polyvalent anti Ig stained 88% of B enriched cells. However, 58% of these cells had IgG bound to Fc receptors, and only 42% had surface Ig stable after brief incubation at 37°C. Further studies are required to determine to what extent the antibodies reacting with such B cell preparations are also heterogeneous.

Original languageEnglish (US)
Pages (from-to)137-139
Number of pages3
JournalTransplantation Proceedings
Volume9
Issue number1 sup.I
Publication statusPublished - 1977

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ASJC Scopus subject areas

  • Surgery
  • Transplantation

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