Adult human hypothalamic tissue was analyzed for the presence of products generated by post-cleavage processing of ACTH 1-13. The displacement curve generated by immunoreactive a-melanocyte stimulating hormone (α-MSHi) in extracts (5 M acetic acid or acidified ethanol) of adult human hypothalamic tissue was parallel to the α-MSH radioimmunoassay standard curve, and α-MSH1 eluted as a single peak on Sephadex G-10, G-25, and G-50 columns in a manner identical to that of synthetic α-MSH1. The α-MSH1 was clearly distinguishable in its mobility on Sephadex G-50 columns from such peptides as α-MSH, luteinizing hormone releasing hormone, and ACTH. After high performance liquid chromatography of extracts of hypothalamic tissue from young as well as aged men and women, we found that the major peak of α-MSH1 (75-95% of total α-MSH1) coeluted with desacetyl α-MSH1(ACTH 1-13 amide) rather than α-MSH. We suggest that desacetyl α-MSH1, rather than α-MSH1, is the predominant α-MSH1 in adult human brain tissue.
- ACTH 1-13 amide
- Adrenocorticotropic hormone
- Desacetyl α-MSH
- High performance liquid chromatography
- Human brain
- Melanocyte stimulating hormone
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