Characterization of in vivo salivary-derived enamel pellicle

I. Al-Hashimi, M. J. Levine

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these methods permitted the identification of individual members of different salivary protein families. The major components of this pellicle were salivary α-amylase, cysteine-containing phosphoprotein (CCP or cystatins), salivary mucin and sIgA. Glycosylated amylase was present in larger quantity than the non-glycosylated species. Only CCPI (cystatin SA-I) of the cysteine-containing phosphoprotein family was identified. The higher molecular-weight salivary mucin (MG1), but not the lower molecular-weight species (MG2), was detected. These results extend earlier observations regarding the selective nature of salivary protein adsorption to enamel surface by demonstrating that only specific members of salivary protein families are involved in 2-h in vivo enamel pellicle formation. The findings also suggest that individual family members may have different functions in the mouth.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalArchives of Oral Biology
Volume34
Issue number4
DOIs
StatePublished - Jan 1 1989

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Dental Pellicle
Salivary Proteins and Peptides
Salivary Cystatins
Polyacrylamide Gel Electrophoresis
Phosphoproteins
Amylases
Mucin-5B
Cysteine
Photofluorography
Mucins
Dental Enamel
Adsorption
Mouth
Glycoproteins
Molecular Weight

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Characterization of in vivo salivary-derived enamel pellicle. / Al-Hashimi, I.; Levine, M. J.

In: Archives of Oral Biology, Vol. 34, No. 4, 01.01.1989, p. 289-295.

Research output: Contribution to journalArticle

Al-Hashimi, I. ; Levine, M. J. / Characterization of in vivo salivary-derived enamel pellicle. In: Archives of Oral Biology. 1989 ; Vol. 34, No. 4. pp. 289-295.
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