Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry

Donald F. Hunt, Robert A. Henderson, Jeffrey Shabanowitz, Kazuyasu Sakaguchi, Hanspeter Michel, Noelle Sevilir, Andrea L. Cox, Ettore Appella, Victor H. Engelhard

Research output: Contribution to journalArticle

951 Citations (Scopus)

Abstract

Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.

Original languageEnglish (US)
Pages (from-to)1261-1263
Number of pages3
JournalScience
Volume255
Issue number5049
StatePublished - Mar 6 1992

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Major Histocompatibility Complex
Mass Spectrometry
Peptides
Electrospray Ionization Mass Spectrometry
Tandem Mass Spectrometry
Autoimmune Diseases
High Pressure Liquid Chromatography
T-Lymphocytes
Antigens
Proteins

ASJC Scopus subject areas

  • General

Cite this

Hunt, D. F., Henderson, R. A., Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., ... Engelhard, V. H. (1992). Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science, 255(5049), 1261-1263.

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. / Hunt, Donald F.; Henderson, Robert A.; Shabanowitz, Jeffrey; Sakaguchi, Kazuyasu; Michel, Hanspeter; Sevilir, Noelle; Cox, Andrea L.; Appella, Ettore; Engelhard, Victor H.

In: Science, Vol. 255, No. 5049, 06.03.1992, p. 1261-1263.

Research output: Contribution to journalArticle

Hunt, DF, Henderson, RA, Shabanowitz, J, Sakaguchi, K, Michel, H, Sevilir, N, Cox, AL, Appella, E & Engelhard, VH 1992, 'Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry', Science, vol. 255, no. 5049, pp. 1261-1263.
Hunt DF, Henderson RA, Shabanowitz J, Sakaguchi K, Michel H, Sevilir N et al. Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science. 1992 Mar 6;255(5049):1261-1263.
Hunt, Donald F. ; Henderson, Robert A. ; Shabanowitz, Jeffrey ; Sakaguchi, Kazuyasu ; Michel, Hanspeter ; Sevilir, Noelle ; Cox, Andrea L. ; Appella, Ettore ; Engelhard, Victor H. / Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. In: Science. 1992 ; Vol. 255, No. 5049. pp. 1261-1263.
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