Chicken Nkx-2.8: A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm

James M. Reecy, Miho Yamada, Kathleen Cummings, Drazen Sosic, Ching Yi Chen, Gregor Eichele, Eric N. Olson, Robert J. Schwartz

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Abstract

Members of the NK family of homeobox transcription factors regulate critical steps of organogenesis during vertebrate development. In the studies described in this report, we have isolated and functionally characterized the chicken Nkx-2.8 (cNkx-2.8) cDNA and protein and defined the temporal and spatial pattern of cNkx-2.8 gene expression during chicken development. cNkx- 2.8 transcripts are first detectable at HH stage 7 in the splanchnopleura. At stage 10+, the cNkx-2.8 gene is expressed in the linear heart tube and the dorsal half of the vitelline vein. However, after looping, HH stage 13, cNkx- 2.8 is no longer expressed in the looped heart tube, but is expressed in the ventral pharyngeal endoderm. At stage 15, in addition to the pharyngeal expression pattern, cNkx-2.8 is expressed in the ectoderm of the pharyngeal arches and the aortic sac. By HH Stage 17, cNkx-2.8 expression is detectable in lateral endoderm of the second and third pharyngeal pouches, the posterior portion of the aortic sac, and the sinus venosus. cNkx-2.8 binds to previously characterized Nkx2-1 and Nkx2-5 DNA-binding sites and overexpression of cNkx-2.8 transactivates a minimal promoter which contains multimerized Nkx-2 DNA-binding sites. In addition, cNkx-2.8 and serum response factor can coactivate a minimal cardiac α-actin promoter. These data are consistent with a model in which cNkx-2.8 performs a unique temporally and spatially restricted function in the developing embryonic heart and pharyngeal region. Moreover, the coexpression of cNkx-2.5 and -2.8 raises the possibility that cNkx-2.8 may have a redundant role with cNkx-2.5 in the coalescing heart tube and may play an important role in the transcriptional program(s) that underlies thymus formation. The existence of multiple NK-2 family members and their partially overlapping patterns of expression are discussed within the framework of a 'Nkx code'.

Original languageEnglish (US)
Pages (from-to)295-311
Number of pages17
JournalDevelopmental Biology
Volume188
Issue number2
DOIs
StatePublished - Aug 15 1997

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Endoderm
Homeobox Genes
Chickens
Stem Cells
Binding Sites
Serum Response Factor
Branchial Region
Sinus of Valsalva
Ectoderm
Organogenesis
DNA
Thymus Gland
Vertebrates
Actins
Veins
Transcription Factors
Complementary DNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Reecy, J. M., Yamada, M., Cummings, K., Sosic, D., Chen, C. Y., Eichele, G., ... Schwartz, R. J. (1997). Chicken Nkx-2.8: A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm. Developmental Biology, 188(2), 295-311. https://doi.org/10.1006/dbio.1997.8641

Chicken Nkx-2.8 : A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm. / Reecy, James M.; Yamada, Miho; Cummings, Kathleen; Sosic, Drazen; Chen, Ching Yi; Eichele, Gregor; Olson, Eric N.; Schwartz, Robert J.

In: Developmental Biology, Vol. 188, No. 2, 15.08.1997, p. 295-311.

Research output: Contribution to journalArticle

Reecy, James M. ; Yamada, Miho ; Cummings, Kathleen ; Sosic, Drazen ; Chen, Ching Yi ; Eichele, Gregor ; Olson, Eric N. ; Schwartz, Robert J. / Chicken Nkx-2.8 : A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm. In: Developmental Biology. 1997 ; Vol. 188, No. 2. pp. 295-311.
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abstract = "Members of the NK family of homeobox transcription factors regulate critical steps of organogenesis during vertebrate development. In the studies described in this report, we have isolated and functionally characterized the chicken Nkx-2.8 (cNkx-2.8) cDNA and protein and defined the temporal and spatial pattern of cNkx-2.8 gene expression during chicken development. cNkx- 2.8 transcripts are first detectable at HH stage 7 in the splanchnopleura. At stage 10+, the cNkx-2.8 gene is expressed in the linear heart tube and the dorsal half of the vitelline vein. However, after looping, HH stage 13, cNkx- 2.8 is no longer expressed in the looped heart tube, but is expressed in the ventral pharyngeal endoderm. At stage 15, in addition to the pharyngeal expression pattern, cNkx-2.8 is expressed in the ectoderm of the pharyngeal arches and the aortic sac. By HH Stage 17, cNkx-2.8 expression is detectable in lateral endoderm of the second and third pharyngeal pouches, the posterior portion of the aortic sac, and the sinus venosus. cNkx-2.8 binds to previously characterized Nkx2-1 and Nkx2-5 DNA-binding sites and overexpression of cNkx-2.8 transactivates a minimal promoter which contains multimerized Nkx-2 DNA-binding sites. In addition, cNkx-2.8 and serum response factor can coactivate a minimal cardiac α-actin promoter. These data are consistent with a model in which cNkx-2.8 performs a unique temporally and spatially restricted function in the developing embryonic heart and pharyngeal region. Moreover, the coexpression of cNkx-2.5 and -2.8 raises the possibility that cNkx-2.8 may have a redundant role with cNkx-2.5 in the coalescing heart tube and may play an important role in the transcriptional program(s) that underlies thymus formation. The existence of multiple NK-2 family members and their partially overlapping patterns of expression are discussed within the framework of a 'Nkx code'.",
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