TY - JOUR
T1 - Chicken Nkx-2.8
T2 - A novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm
AU - Reecy, James M.
AU - Yamada, Miho
AU - Cummings, Kathleen
AU - Sosic, Drazen
AU - Chen, Ching Yi
AU - Eichele, Gregor
AU - Olson, Eric N.
AU - Schwartz, Robert J.
N1 - Funding Information:
This study was supported by NIH R01 HL50422 and P01 HL49953. The authors thank Dr. Margaret L. Kirby for her assistance in anatomy identi®cation.
PY - 1997/8/15
Y1 - 1997/8/15
N2 - Members of the NK family of homeobox transcription factors regulate critical steps of organogenesis during vertebrate development. In the studies described in this report, we have isolated and functionally characterized the chicken Nkx-2.8 (cNkx-2.8) cDNA and protein and defined the temporal and spatial pattern of cNkx-2.8 gene expression during chicken development. cNkx- 2.8 transcripts are first detectable at HH stage 7 in the splanchnopleura. At stage 10+, the cNkx-2.8 gene is expressed in the linear heart tube and the dorsal half of the vitelline vein. However, after looping, HH stage 13, cNkx- 2.8 is no longer expressed in the looped heart tube, but is expressed in the ventral pharyngeal endoderm. At stage 15, in addition to the pharyngeal expression pattern, cNkx-2.8 is expressed in the ectoderm of the pharyngeal arches and the aortic sac. By HH Stage 17, cNkx-2.8 expression is detectable in lateral endoderm of the second and third pharyngeal pouches, the posterior portion of the aortic sac, and the sinus venosus. cNkx-2.8 binds to previously characterized Nkx2-1 and Nkx2-5 DNA-binding sites and overexpression of cNkx-2.8 transactivates a minimal promoter which contains multimerized Nkx-2 DNA-binding sites. In addition, cNkx-2.8 and serum response factor can coactivate a minimal cardiac α-actin promoter. These data are consistent with a model in which cNkx-2.8 performs a unique temporally and spatially restricted function in the developing embryonic heart and pharyngeal region. Moreover, the coexpression of cNkx-2.5 and -2.8 raises the possibility that cNkx-2.8 may have a redundant role with cNkx-2.5 in the coalescing heart tube and may play an important role in the transcriptional program(s) that underlies thymus formation. The existence of multiple NK-2 family members and their partially overlapping patterns of expression are discussed within the framework of a 'Nkx code'.
AB - Members of the NK family of homeobox transcription factors regulate critical steps of organogenesis during vertebrate development. In the studies described in this report, we have isolated and functionally characterized the chicken Nkx-2.8 (cNkx-2.8) cDNA and protein and defined the temporal and spatial pattern of cNkx-2.8 gene expression during chicken development. cNkx- 2.8 transcripts are first detectable at HH stage 7 in the splanchnopleura. At stage 10+, the cNkx-2.8 gene is expressed in the linear heart tube and the dorsal half of the vitelline vein. However, after looping, HH stage 13, cNkx- 2.8 is no longer expressed in the looped heart tube, but is expressed in the ventral pharyngeal endoderm. At stage 15, in addition to the pharyngeal expression pattern, cNkx-2.8 is expressed in the ectoderm of the pharyngeal arches and the aortic sac. By HH Stage 17, cNkx-2.8 expression is detectable in lateral endoderm of the second and third pharyngeal pouches, the posterior portion of the aortic sac, and the sinus venosus. cNkx-2.8 binds to previously characterized Nkx2-1 and Nkx2-5 DNA-binding sites and overexpression of cNkx-2.8 transactivates a minimal promoter which contains multimerized Nkx-2 DNA-binding sites. In addition, cNkx-2.8 and serum response factor can coactivate a minimal cardiac α-actin promoter. These data are consistent with a model in which cNkx-2.8 performs a unique temporally and spatially restricted function in the developing embryonic heart and pharyngeal region. Moreover, the coexpression of cNkx-2.5 and -2.8 raises the possibility that cNkx-2.8 may have a redundant role with cNkx-2.5 in the coalescing heart tube and may play an important role in the transcriptional program(s) that underlies thymus formation. The existence of multiple NK-2 family members and their partially overlapping patterns of expression are discussed within the framework of a 'Nkx code'.
UR - http://www.scopus.com/inward/record.url?scp=0031571635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031571635&partnerID=8YFLogxK
U2 - 10.1006/dbio.1997.8641
DO - 10.1006/dbio.1997.8641
M3 - Article
C2 - 9268576
AN - SCOPUS:0031571635
SN - 0012-1606
VL - 188
SP - 295
EP - 311
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -