Chromatin Immunoprecipitation and Microarray Analysis Suggest Functional Cooperation between Kaposi's Sarcoma-Associated Herpesvirus ORF57 and K-bZIP

Olga V. Hunter, Emi Sei, R. Blake Richardson, Nicholas K. Conrad

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated in nearly every aspect of viral gene expression, including transcription, RNA stability, splicing, export, and translation. Here we demonstrate that ORF57 interacts with the KSHV K-bZIP protein in vitro and in cell extracts from lytically reactivated infected cells. To further test the biological relevance of the interaction, we performed a chromatin immunoprecipitation and microarray (ChIP-chip) analysis using anti-ORF57 antibodies and a KSHV tiling array. The results revealed four specific areas of enrichment, including the ORF4 and K8 (K-bZIP) promoters, as well as oriLyt, all of which interact with K-bZIP. In addition, ORF57 associated with DNA corresponding to the PAN RNA transcribed region, a known posttranscriptional target of ORF57. All of the peaks were RNase insensitive, demonstrating that ORF57 association with the viral genome is unlikely to be mediated exclusively by an RNA tether. Our data demonstrate that ORF57 associates with the viral genome by using at least two modes of recruitment, and they suggest that ORF57 and K-bZIP coregulate viral gene expression during lytic infection.

Original languageEnglish (US)
Pages (from-to)4005-4016
Number of pages12
JournalJournal of Virology
Volume87
Issue number7
DOIs
StatePublished - Apr 2013

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Human herpesvirus 8
Human Herpesvirus 8
Chromatin Immunoprecipitation
Microarray Analysis
Open Reading Frames
chromatin
open reading frames
Viral Genes
Viral Genome
RNA
Gene Expression
gene expression
Basic-Leucine Zipper Transcription Factors
RNA Splicing
Herpesviridae Infections
genome
RNA Stability
Regulator Genes
Ribonucleases
virus replication

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Chromatin Immunoprecipitation and Microarray Analysis Suggest Functional Cooperation between Kaposi's Sarcoma-Associated Herpesvirus ORF57 and K-bZIP. / Hunter, Olga V.; Sei, Emi; Blake Richardson, R.; Conrad, Nicholas K.

In: Journal of Virology, Vol. 87, No. 7, 04.2013, p. 4005-4016.

Research output: Contribution to journalArticle

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