Chromosomal imbalances in the colorectal carcinomas with microsatellite instability

Long Shan Li, Nam Gyun Kim, Se Hoon Kim, Chanil Park, Hyunki Kim, Hyun Ju Kang, Kwi Hye Koh, Soo Nyung Kim, Won Ho Kim, Nam Kyu Kim, Hoguen Kim

Research output: Contribution to journalArticle

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Abstract

Recent molecular genetic studies have revealed that two major types of genomic instabilities, chromosomal instability and microsatellite instability, exist in the colorectal carcinomas. To clarify the relationship between chromosomal abnormalities and mismatch repair gene defects in colorectal carcinomas, we performed a chromosomal analysis on 39 colorectal carcinomas with high-microsatellite instability (MSI-H) and compared the results obtained with those of 20 right-sided microsatellite-stable (MSS) colorectal carcinomas. Chromosomal imbalances (CIs) in MSS colorectal carcinomas were more frequent than in MSI-H colon carcinomas by comparative genomic hybridization analysis (70% versus 31%, P = 0.004). The CI patterns of MSI-H and MSS carcinomas were different. Frequent CIs in MSI-H colon carcinomas were gains of 4q (15%) and 8q (8%), and losses of 9q (21%), 1p (18%), and 11q (18%). In contrast, frequent CIs in right-sided MSS colon carcinomas were gains of 8q (50%), 13q (35%), and 20q (25%), and losses of 18q (55%), 15q (35%), and 17p (30%). We compared the mutation status of 45 target genes and CIs in our MSI-H tumors. Among these 45 target genes, mutation of hRAD50, a member of the DNA repair genes, and FLJ11383 were significantly related to MSI-H colorectal carcinomas with CIs (P = 0.01 and P = 0.02, respectively). Our findings indicate that unique CIs were present in a subset of MSI-H colorectal carcinomas and that these CIs are related to the mutation of several target genes, especially of hRAD50.

Original languageEnglish (US)
Pages (from-to)1429-1436
Number of pages8
JournalAmerican Journal of Pathology
Volume163
Issue number4
StatePublished - Oct 1 2003

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Microsatellite Instability
Colorectal Neoplasms
Microsatellite Repeats
Carcinoma
Colon
Genes
Mutation
Chromosomal Instability
DNA Mismatch Repair
Comparative Genomic Hybridization
Genomic Instability
N-methylsuccinimide
Chromosome Aberrations
DNA Repair
Molecular Biology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Li, L. S., Kim, N. G., Kim, S. H., Park, C., Kim, H., Kang, H. J., ... Kim, H. (2003). Chromosomal imbalances in the colorectal carcinomas with microsatellite instability. American Journal of Pathology, 163(4), 1429-1436.

Chromosomal imbalances in the colorectal carcinomas with microsatellite instability. / Li, Long Shan; Kim, Nam Gyun; Kim, Se Hoon; Park, Chanil; Kim, Hyunki; Kang, Hyun Ju; Koh, Kwi Hye; Kim, Soo Nyung; Kim, Won Ho; Kim, Nam Kyu; Kim, Hoguen.

In: American Journal of Pathology, Vol. 163, No. 4, 01.10.2003, p. 1429-1436.

Research output: Contribution to journalArticle

Li, LS, Kim, NG, Kim, SH, Park, C, Kim, H, Kang, HJ, Koh, KH, Kim, SN, Kim, WH, Kim, NK & Kim, H 2003, 'Chromosomal imbalances in the colorectal carcinomas with microsatellite instability', American Journal of Pathology, vol. 163, no. 4, pp. 1429-1436.
Li LS, Kim NG, Kim SH, Park C, Kim H, Kang HJ et al. Chromosomal imbalances in the colorectal carcinomas with microsatellite instability. American Journal of Pathology. 2003 Oct 1;163(4):1429-1436.
Li, Long Shan ; Kim, Nam Gyun ; Kim, Se Hoon ; Park, Chanil ; Kim, Hyunki ; Kang, Hyun Ju ; Koh, Kwi Hye ; Kim, Soo Nyung ; Kim, Won Ho ; Kim, Nam Kyu ; Kim, Hoguen. / Chromosomal imbalances in the colorectal carcinomas with microsatellite instability. In: American Journal of Pathology. 2003 ; Vol. 163, No. 4. pp. 1429-1436.
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