Chromosome 7 aneusomy in metaplastic breast carcinomas with chondroid, squamous, and spindle-cell differentiation

Katja Gwin, Kimberly Lezon-Geyda, Lyndsay Harris, Fattaneh A. Tavassoli

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metaplastic breast carcinomas (MBCs) are basal-like tumors that frequently express epidermal growth factor receptor (EGFR) via an unknown underlying genetic mechanism. In this study, the EGFR/CEP7 gene copy number in 17 MBCs with chondroid, squamous, and spindle-cell differentiation showing EGFR expression by immunohistochemistry was analyzed using fluorescence in situ hybridization. All cases had a balanced EGFR/CEP7 ratio. EGFR gene amplification was not observed in any case. Monosomy was found in 25% and polysomy in 12.5% of carcinomas with chondroid differentiation. All spindle-cell carcinomas and 50% of squamous carcinomas showed trisomy. Comparison with CEP7 copy number revealed aneusomy of chromosome 7, as opposed to increases or decreases specific to the EGFR gene or 7p. Although no direct correlation between EGFR expression by immunohistochemistry and aneusomy was observed, cases with a score of 3+ showed a higher frequency of EGFR gene copy gain. In the absence of EGFR amplification, chromosome 7 aneusomy might be a useful criterion for the determination of potential candidates for EGFR tyrosine kinase inhibitor clinical trials.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalInternational Journal of Surgical Pathology
Volume19
Issue number1
DOIs
StatePublished - Feb 2011

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Chromosomes, Human, Pair 7
Epidermal Growth Factor Receptor
erbB-1 Genes
Cell Differentiation
Epithelial Cells
Breast Neoplasms
Immunohistochemistry
Carcinoma
Monosomy
Gene Dosage
Gene Amplification
Trisomy
Fluorescence In Situ Hybridization
Protein-Tyrosine Kinases
Squamous Cell Carcinoma
Clinical Trials
Neoplasms

Keywords

  • basal-like tumors
  • breast
  • carcinoma
  • EGFR
  • gene copy number
  • metaplastic

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

Chromosome 7 aneusomy in metaplastic breast carcinomas with chondroid, squamous, and spindle-cell differentiation. / Gwin, Katja; Lezon-Geyda, Kimberly; Harris, Lyndsay; Tavassoli, Fattaneh A.

In: International Journal of Surgical Pathology, Vol. 19, No. 1, 02.2011, p. 20-25.

Research output: Contribution to journalArticle

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