Circulating glucose levels inversely correlate with Drosophila larval feeding through insulin signaling and SLC5A11

Rupali Ugrankar, Pano Theodoropoulos, Fatih Akdemir, William M Henne, Jonathan M Graff

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.

Original languageEnglish (US)
Article number110
JournalCommunications Biology
Volume1
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Drosophila
insulin
Insulin
Glucose
glucose
Larva
Trehalose
larvae
Feeding Behavior
hyperglycemia
trehalose
appetite
food choices
Food
Hyperglycemia
feeding behavior
Insulins
Appetite Regulation
Mammals
Hemolymph

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Circulating glucose levels inversely correlate with Drosophila larval feeding through insulin signaling and SLC5A11. / Ugrankar, Rupali; Theodoropoulos, Pano; Akdemir, Fatih; Henne, William M; Graff, Jonathan M.

In: Communications Biology, Vol. 1, No. 1, 110, 01.12.2018.

Research output: Contribution to journalArticle

@article{acd5005066e94762af52f540515383cb,
title = "Circulating glucose levels inversely correlate with Drosophila larval feeding through insulin signaling and SLC5A11",
abstract = "In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.",
author = "Rupali Ugrankar and Pano Theodoropoulos and Fatih Akdemir and Henne, {William M} and Graff, {Jonathan M}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s42003-018-0109-4",
language = "English (US)",
volume = "1",
journal = "Communications Biology",
issn = "2399-3642",
number = "1",

}

TY - JOUR

T1 - Circulating glucose levels inversely correlate with Drosophila larval feeding through insulin signaling and SLC5A11

AU - Ugrankar, Rupali

AU - Theodoropoulos, Pano

AU - Akdemir, Fatih

AU - Henne, William M

AU - Graff, Jonathan M

PY - 2018/12/1

Y1 - 2018/12/1

N2 - In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.

AB - In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.

UR - http://www.scopus.com/inward/record.url?scp=85071155913&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071155913&partnerID=8YFLogxK

U2 - 10.1038/s42003-018-0109-4

DO - 10.1038/s42003-018-0109-4

M3 - Article

VL - 1

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 110

ER -