CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules

Shuang Shi, Min Rui, Wenling Han, Ying Wang, Xiaoyan Qiu, Peiguo Ding, Pei Zhang, Xiaohui Zhu, Yingmei Zhang, Qini Gan, Dalong Ma

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8 kb on chromosome 16q22.1. Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis.

Original languageEnglish (US)
Pages (from-to)1633-1640
Number of pages8
JournalInternational Journal of Biochemistry and Cell Biology
Volume37
Issue number8
DOIs
StatePublished - Aug 2005

Keywords

  • CKLFSF
  • CKLFSF2
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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