Clonality of combined tumors

A molecular genetic study

Jiaoti Huang, Carmen Behrens, Ignacio I. Wistuba, Adi F. Gazdar, Jaishree Jagirdar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Context. - Tumors with mixed morphologic patterns (combined tumors) are sometimes encountered, and questions often arise regarding the mechanism of molecular pathogenesis of each component and their relationships. Objective. - To determine whether different components of combined tumors contain the same or different genetic alterations, thus providing evidence for their clonality. Materials and Methods. - Six combined tumors with 2 components (in each case, both components showed epithelial differentiation morphologically) were studied by microdissecting tumor cells from each morphologic area followed by loss of heterozygosity analysis. Results. - In 1 of the cases studied, the different morphologic areas contained different patterns of genetic alterations. In the remaining 5 cases, the different morphologic areas harbored identical genetic changes in the chromosome regions studied. The latter group, interestingly, included a colonic tumor with an area of tubulovillous adenoma and an area of neuroendocrine carcinoma, and 2 lung tumors with squamous carcinoma and small cell carcinoma components. Conclusions. - Our results suggest that in the majority of combined tumors, cells with different phenotypes share similar genotype and may arise from a single precursor cell. However, in a minority of these tumors, different areas may be derived from different precursor cells.

Original languageEnglish (US)
Pages (from-to)437-441
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume126
Issue number4
StatePublished - 2002

Fingerprint

Molecular Biology
Neoplasms
Neuroendocrine Carcinoma
Small Cell Carcinoma
Loss of Heterozygosity
Cellular Structures
Adenoma
Squamous Cell Carcinoma
Chromosomes
Genotype
Phenotype
Lung

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Huang, J., Behrens, C., Wistuba, I. I., Gazdar, A. F., & Jagirdar, J. (2002). Clonality of combined tumors: A molecular genetic study. Archives of Pathology and Laboratory Medicine, 126(4), 437-441.

Clonality of combined tumors : A molecular genetic study. / Huang, Jiaoti; Behrens, Carmen; Wistuba, Ignacio I.; Gazdar, Adi F.; Jagirdar, Jaishree.

In: Archives of Pathology and Laboratory Medicine, Vol. 126, No. 4, 2002, p. 437-441.

Research output: Contribution to journalArticle

Huang, J, Behrens, C, Wistuba, II, Gazdar, AF & Jagirdar, J 2002, 'Clonality of combined tumors: A molecular genetic study', Archives of Pathology and Laboratory Medicine, vol. 126, no. 4, pp. 437-441.
Huang, Jiaoti ; Behrens, Carmen ; Wistuba, Ignacio I. ; Gazdar, Adi F. ; Jagirdar, Jaishree. / Clonality of combined tumors : A molecular genetic study. In: Archives of Pathology and Laboratory Medicine. 2002 ; Vol. 126, No. 4. pp. 437-441.
@article{94c26905d81d4cf1a6f4a77bfbe0374d,
title = "Clonality of combined tumors: A molecular genetic study",
abstract = "Context. - Tumors with mixed morphologic patterns (combined tumors) are sometimes encountered, and questions often arise regarding the mechanism of molecular pathogenesis of each component and their relationships. Objective. - To determine whether different components of combined tumors contain the same or different genetic alterations, thus providing evidence for their clonality. Materials and Methods. - Six combined tumors with 2 components (in each case, both components showed epithelial differentiation morphologically) were studied by microdissecting tumor cells from each morphologic area followed by loss of heterozygosity analysis. Results. - In 1 of the cases studied, the different morphologic areas contained different patterns of genetic alterations. In the remaining 5 cases, the different morphologic areas harbored identical genetic changes in the chromosome regions studied. The latter group, interestingly, included a colonic tumor with an area of tubulovillous adenoma and an area of neuroendocrine carcinoma, and 2 lung tumors with squamous carcinoma and small cell carcinoma components. Conclusions. - Our results suggest that in the majority of combined tumors, cells with different phenotypes share similar genotype and may arise from a single precursor cell. However, in a minority of these tumors, different areas may be derived from different precursor cells.",
author = "Jiaoti Huang and Carmen Behrens and Wistuba, {Ignacio I.} and Gazdar, {Adi F.} and Jaishree Jagirdar",
year = "2002",
language = "English (US)",
volume = "126",
pages = "437--441",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "4",

}

TY - JOUR

T1 - Clonality of combined tumors

T2 - A molecular genetic study

AU - Huang, Jiaoti

AU - Behrens, Carmen

AU - Wistuba, Ignacio I.

AU - Gazdar, Adi F.

AU - Jagirdar, Jaishree

PY - 2002

Y1 - 2002

N2 - Context. - Tumors with mixed morphologic patterns (combined tumors) are sometimes encountered, and questions often arise regarding the mechanism of molecular pathogenesis of each component and their relationships. Objective. - To determine whether different components of combined tumors contain the same or different genetic alterations, thus providing evidence for their clonality. Materials and Methods. - Six combined tumors with 2 components (in each case, both components showed epithelial differentiation morphologically) were studied by microdissecting tumor cells from each morphologic area followed by loss of heterozygosity analysis. Results. - In 1 of the cases studied, the different morphologic areas contained different patterns of genetic alterations. In the remaining 5 cases, the different morphologic areas harbored identical genetic changes in the chromosome regions studied. The latter group, interestingly, included a colonic tumor with an area of tubulovillous adenoma and an area of neuroendocrine carcinoma, and 2 lung tumors with squamous carcinoma and small cell carcinoma components. Conclusions. - Our results suggest that in the majority of combined tumors, cells with different phenotypes share similar genotype and may arise from a single precursor cell. However, in a minority of these tumors, different areas may be derived from different precursor cells.

AB - Context. - Tumors with mixed morphologic patterns (combined tumors) are sometimes encountered, and questions often arise regarding the mechanism of molecular pathogenesis of each component and their relationships. Objective. - To determine whether different components of combined tumors contain the same or different genetic alterations, thus providing evidence for their clonality. Materials and Methods. - Six combined tumors with 2 components (in each case, both components showed epithelial differentiation morphologically) were studied by microdissecting tumor cells from each morphologic area followed by loss of heterozygosity analysis. Results. - In 1 of the cases studied, the different morphologic areas contained different patterns of genetic alterations. In the remaining 5 cases, the different morphologic areas harbored identical genetic changes in the chromosome regions studied. The latter group, interestingly, included a colonic tumor with an area of tubulovillous adenoma and an area of neuroendocrine carcinoma, and 2 lung tumors with squamous carcinoma and small cell carcinoma components. Conclusions. - Our results suggest that in the majority of combined tumors, cells with different phenotypes share similar genotype and may arise from a single precursor cell. However, in a minority of these tumors, different areas may be derived from different precursor cells.

UR - http://www.scopus.com/inward/record.url?scp=0036217252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036217252&partnerID=8YFLogxK

M3 - Article

VL - 126

SP - 437

EP - 441

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 4

ER -