TY - JOUR
T1 - Cloning and Characterization of ELL-associated Proteins EAP45 and EAP20
T2 - A role for yeast EAP-like proteins in regulation of gene expression by glucose
AU - Kamura, Takumi
AU - Burian, Dennis
AU - Khalili, Hamed
AU - Schmidt, Susan L.
AU - Sato, Shigeo
AU - Liu, Wen Jun
AU - Conrad, Michael N.
AU - Conaway, Ronald C.
AU - Conaway, Joan Weliky
AU - Shilatifard, Ali
PY - 2001/5/11
Y1 - 2001/5/11
N2 - RNA polymerase II elongation factor ELL was recently purified from rat liver as a component of a multiprotein complex containing ELL and three ELL-associated proteins (EAPS) of ∼45 (EAP45), ∼30 (EAP30), and ∼20 (EAP20) kDa (Shilatifard, A. (1998) J. Biol. Chem. 273, 11212-11217). Cloning of cDNA encoding the EAP30 protein revealed that it shares significant sequence similarity with the product of the Saccharomyces cerevisiae SNF8 gene (Schmidt, A. E., Miller, T., Schmidt, S. L., Shiekhattar, R., and Shilatifard, A. (1999) J. Biol. Chem. 274, 21981-21985), which is required for efficient derepression of glucose-repressed genes. Here we report the cloning of cDNAs encoding the EAP45 and EAP20 proteins. In addition, we identify the S. cerevisiae VPS36 and YJR102c genes as potential orthologs of EAP45 and EAP20 and show that they are previously uncharacterized SNF genes with properties very similar to SNF8.
AB - RNA polymerase II elongation factor ELL was recently purified from rat liver as a component of a multiprotein complex containing ELL and three ELL-associated proteins (EAPS) of ∼45 (EAP45), ∼30 (EAP30), and ∼20 (EAP20) kDa (Shilatifard, A. (1998) J. Biol. Chem. 273, 11212-11217). Cloning of cDNA encoding the EAP30 protein revealed that it shares significant sequence similarity with the product of the Saccharomyces cerevisiae SNF8 gene (Schmidt, A. E., Miller, T., Schmidt, S. L., Shiekhattar, R., and Shilatifard, A. (1999) J. Biol. Chem. 274, 21981-21985), which is required for efficient derepression of glucose-repressed genes. Here we report the cloning of cDNAs encoding the EAP45 and EAP20 proteins. In addition, we identify the S. cerevisiae VPS36 and YJR102c genes as potential orthologs of EAP45 and EAP20 and show that they are previously uncharacterized SNF genes with properties very similar to SNF8.
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U2 - 10.1074/jbc.M010142200
DO - 10.1074/jbc.M010142200
M3 - Article
C2 - 11278625
AN - SCOPUS:0035844303
SN - 0021-9258
VL - 276
SP - 16528
EP - 16533
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 19
ER -